Mouse CD31 ELISA Kit
Cat no : KE10157
Synonyms
Pecam, PECAM 1, Pecam1, PECAM-1
Validation Data Gallery
Product Information
KE10157 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse CD31 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse CD31. The assay recognizes mouse CD31. An antibody specific for mouse CD31 has been pre-coated onto the microwells. The mouse CD31 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for mouse CD31 is added to detect the captured mouse CD31 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse CD31 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell lysate, Tissue lysate |
| Assay type | Sandwich |
| Sensitivity | 0.8 pg/mL |
| Range | 19.5-1250 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 18613 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 92% | 87%-98% |
| Tissue lysate | 99% | 94%-105% |
| Mouse serum | 95% | 84%-118% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 669.0 | 30.3 | 4.5 |
| 2 | 8 | 181.7 | 6.5 | 3.6 |
| 3 | 8 | 49.3 | 3.5 | 7.0 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 676.3 | 25.0 | 3.7 |
| 2 | 16 | 182.2 | 10.8 | 5.9 |
| 3 | 16 | 51.4 | 3.8 | 7.4 |
Background Information
CD31, also known as platelet endothelial cell adhesion molecule1 (PECAM-1), is a 130 kDa platelet-endothelial cell (EC) adhesion molecule initially identified from ECs and platelets and later from blood leucocytes. CD31 was initially classified as a cell adhesion molecule, later studies suggested that CD31 triggers downstream inhibitory signaling upon trans-homophilic CD31 engagement during cell-cell interaction. CD31 signaling participates in regulating leucocyte detachment, T-cell activation, platelet activation, and angiogenesis, all of which are critical to the pathogenesis of atherosclerosis and abdominal aortic aneurysms (AAAs).
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Pecam, PECAM 1, Pecam1, PECAM-1 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.