Mouse CD40 ELISA Kit
Cat no : KE10196
Synonyms
Bp50, CD40 antigen, Tnfrsf5
Validation Data Gallery
Product Information
KE10196 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse CD40 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse CD40. The assay recognizes mouse CD40. An antibody specific for mouse CD40 has been pre-coated onto the microwells. The mouse CD40 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for mouse CD40 is added to detect the captured mouse CD40 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse CD40 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant, Tissue lysate |
| Assay type | Sandwich |
| Sensitivity | 2.8 pg/mL |
| Range | 31.25-2000 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 21939 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 78% | 77%-81% |
| Cell culture supernatant | 96% | 87%-111% |
| Tissue lysate | 79% | 72%-88% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 1,113.4 | 40.9 | 3.7 |
| 2 | 8 | 264.9 | 9.3 | 3.5 |
| 3 | 8 | 132.7 | 3.7 | 2.8 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 1,129.8 | 41.2 | 3.6 |
| 2 | 16 | 268.9 | 10.2 | 3.8 |
| 3 | 16 | 132.7 | 4.0 | 3.0 |
Background Information
CD40, also named as TNFRSF5 and Bp50, is a type I transmembrane protein that belongs to the tumor necrosis factor receptor (TNFR) family. CD40 is expressed on B cells, dendritic cells (DCs), monocytes, platelets, and macrophages as well as by non-hematopoietic cells such as myofibroblasts, fibroblasts, epithelial, and endothelial cells. It is a receptor for TNFSF5/CD40L. CD40L/CD40 interactions are essential for immunoglobulin (Ig) isotype switching, germinal center formation and the development of B cell memory.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Bp50, CD40 antigen, Tnfrsf5 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.