Speedy™ Mouse CD40 One-Step ELISA Kit
Cat no : SE60035
Synonyms
Bp50, CD40 antigen, Tnfrsf5
Validation Data Gallery
Product Information
| Product name | Speedy™ Mouse CD40 One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant, Tissue lysate |
| Assay type | Sandwich |
| Sensitivity | 2.5 pg/mL |
| Range | 31.25-2000 pg/mL, 15.6-1000 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 21939 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 104% | 83%-125% |
| Cell culture supernatant | 87% | 75%-98% |
| Tissue lysate | 89% | 81%-99% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 650.6 | 31.5 | 4.8 |
| 2 | 8 | 165.6 | 3.6 | 2.2 |
| 3 | 8 | 87.5 | 3.3 | 3.8 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 623.4 | 37.1 | 6.0 |
| 2 | 16 | 159.1 | 7.6 | 4.8 |
| 3 | 16 | 88.5 | 6.1 | 6.9 |
Background Information
CD40, also named as TNFRSF5 and Bp50, is a type I transmembrane protein that belongs to the tumor necrosis factor receptor (TNFR) family. CD40 is expressed on B cells, dendritic cells (DCs), monocytes, platelets, and macrophages as well as by non-hematopoietic cells such as myofibroblasts, fibroblasts, epithelial, and endothelial cells. It is a receptor for TNFSF5/CD40L. CD40L/CD40 interactions are essential for immunoglobulin (Ig) isotype switching, germinal center formation and the development of B cell memory.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Bp50, CD40 antigen, Tnfrsf5 |
FAQ
1、If thermostatic shaker is not available, will it affect the duration of my experiment and the experimental data?
Answer: If thermostatic shaker is not available, it may take 2.5 hours to complete this experiment. After adding the antibody cocktail, we suggest you incubate the plate at 37℃ for 2 hours. After adding TMB, you only need to incubate the plate at 37℃ for 15 minutes. Through comparative experiments, our R&D personnel have confirmed that you can still obtain valid experimental data, although the signal will be slightly reduced.