Speedy™ Mouse CD40 One-Step ELISA Kit

Sensitivity

2.5 pg/mL

Range

31.25-2000 pg/mL, 15.6-1000 pg/mL

Reactivity

Mouse

Cat no : SE60035

Synonyms

Bp50, CD40 antigen, Tnfrsf5



Product Information

Product name Speedy™ Mouse CD40 One-Step ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, Plasma, Cell culture supernatant, Tissue lysate
Assay type Sandwich
Sensitivity 2.5 pg/mL
Range 31.25-2000 pg/mL, 15.6-1000 pg/mL
Reactivity Mouse
Tested applications Sandwich ELISA
Gene ID (NCBI) 21939

Recovery

Sample TypeAverageRange
Mouse serum104%83%-125%
Cell culture supernatant87%75%-98%
Tissue lysate89%81%-99%

IntraAssay

Samplenmean ( pg/mL)SDCV%
18650.631.54.8
28165.63.62.2
3887.53.33.8

InterAssay

Samplenmean ( pg/mL)SDCV%
116623.437.16.0
216159.17.64.8
31688.56.16.9

Background Information

CD40, also named as TNFRSF5 and Bp50, is a type I transmembrane protein that belongs to the tumor necrosis factor receptor (TNFR) family. CD40 is expressed on B cells, dendritic cells (DCs), monocytes, platelets, and macrophages as well as by non-hematopoietic cells such as myofibroblasts, fibroblasts, epithelial, and endothelial cells. It is a receptor for TNFSF5/CD40L. CD40L/CD40 interactions are essential for immunoglobulin (Ig) isotype switching, germinal center formation and the development of B cell memory.

Properties

Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
Synonyms Bp50, CD40 antigen, Tnfrsf5
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