Mouse CD6 ELISA Kit
Cat no : KE10160
Synonyms
T-cell differentiation antigen CD6
Validation Data Gallery
Product Information
KE10160 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse CD6 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse CD6. The assay recognizes mouse CD6. An antibody specific for mouse CD6 has been pre-coated onto the microwells. The mouse CD6 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for mouse CD6 is added to detect the captured mouse CD6 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse CD6 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma |
| Assay type | Sandwich |
| Sensitivity | 12.0 pg/mL |
| Range | 78.1-5000 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 12511 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 99% | 95%-106% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 2,316.7 | 135.6 | 5.9 |
| 2 | 8 | 445.0 | 27.1 | 6.1 |
| 3 | 8 | 211.6 | 15.6 | 7.4 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 2,403.4 | 179.5 | 7.5 |
| 2 | 16 | 469.6 | 44.6 | 9.5 |
| 3 | 16 | 221.1 | 22.2 | 10.1 |
Background Information
The transmembrane surface glycoprotein CD6 is a receptor expressed on thymocytes, mature T cells, the B1a B cell subset, and certain regions of the brain. It is a marker of T cells and an important T-cell regulator. CD6 acts as a costimulatory molecule synergizing with TCR or CD28 to enhance T cell proliferation. CD6 variants have been identified as susceptibility or disease modifier markers for multiple sclerosis, inflammatory bowel disease, Behçe's disease, psoriasis, and rheumatoid arthritis. Besides, Mouse soluble CD6 may have antibacterial effects, which can be used as adjunctive therapy in experimental models of complex and severe polymicrobial sepsis.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | T-cell differentiation antigen CD6 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.