Mouse EPO/Erythropoietin ELISA Kit
Cat no : KE10031
Synonyms
Epo, erythropoietin
Validation Data Gallery
Product Information
KE10031 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Erythropoietin ELISA kit is to be used to detect and quantify protein levels of endogenous Erythropoietin. The assay recognizes mouse Erythropoietin. An antibody specific for Erythropoietin has been pre-coated onto the microwells. The Erythropoietin protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouse Erythropoietin is added to detect the captured mouse Erythropoietin protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse EPO/Erythropoietin ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 12 pg/mL |
| Range | 78.1-5000 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 13856 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 90% | 71%-117% |
| cell culture supernatants | 98% | 70%-119% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 252.2 | 20.7 | 8.2 |
| 2 | 20 | 1,053.8 | 47.4 | 4.5 |
| 3 | 20 | 4,276.6 | 197.0 | 4.6 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 267.7 | 22.8 | 8.5 |
| 2 | 24 | 1,074.6 | 48.0 | 4.5 |
| 3 | 24 | 4,481.0 | 204.8 | 4.6 |
Background Information
Erythropoietin (EPO) is a glycoprotein hormone that regulates the production of red blood cells and biosynthesis of hemoglobin. The predominant expression of this gene shifts from the liver during fetal development to kidney in adults, and and the secreted protein will travel through the blood stream to reach to the bone marrow to stimulate hematopoietic stem cell differentiation to RBC. EPO binds to the cognate EPO receptor (EPOR) on erythroid progenitor cells, thus preventing apoptosis and stimulating their differentiation and maturation into erythrocyte. However, EPO protein and its receptors have also been shown to be cytoprotective in extra-hematopoietic tissues including the retina tissue. A complete lack of erythropoietin causes embryonic lethal anemia in mice. The conditional inactivation of erythropoietin in adult mice results in a chronic, normocytic and normochromic anemia.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Epo, erythropoietin |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
Publications
| Species | Sample Type | Title |
|---|---|---|
Nutrition Changes in iron metabolism centered on hepcidin due to high-intensity exercise under restricted food intake. | ||
ACS Pharmacol Transl Sci Prolyl Hydroxylase Domain Protein Inhibitor Not Harboring a 2-Oxoglutarate Scaffold Protects against Hypoxic Stress. | ||
Physiol Rep Pharmacological prolyl hydroxylase inhibition enhances high-intensity exercise capacity in mice | ||
Lipids Health Dis Deficiency of lysophosphatidic acid receptor 3 decreases erythropoietin production in hypoxic mouse kidneys | ||
Research (Wash D C) Directed Evolution of T7 RNA Polymerase Minimizes dsRNA By-product and Enables High-Fidelity mRNA Synthesis for Demanding Therapeutic Applications. |