Speedy™ Mouse Foxp3 One-Step ELISA Kit
Cat no : SE60063
Synonyms
forkhead box P3, Forkhead box protein P3 41 kDa form, Forkhead box protein P3, C-terminally processed, JM2, scurfin
Validation Data Gallery
Product Information
| Product name | Speedy™ Mouse Foxp3 One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Tissue lysate |
| Assay type | Sandwich |
| Sensitivity | 6.6 pg/mL |
| Range | 39-2500 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 20371 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Tissue lysate | 86% | 81%-89% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 1,230.6 | 23.5 | 1.9 |
| 2 | 8 | 307.8 | 5.6 | 1.8 |
| 3 | 8 | 144.0 | 5.0 | 3.5 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 1,237.7 | 26.4 | 2.1 |
| 2 | 16 | 315.0 | 11.2 | 3.6 |
| 3 | 16 | 152.3 | 10.5 | 6.9 |
Background Information
Foxp3 (Forkhead box P3) is a transcription factor that belongs to the Forkhead box family. It is a key transcription factor for regulatory T cells (Tregs) and is crucial for maintaining immune system homeostasis. Deficiency in Foxp3 can lead to immune dysregulation, polyendocrinopathy, enteropathy, and X-linked syndrome (IPEX). In mice, the expression of Foxp3 is primarily localized in the nuclei of regulatory T cells. This protein maintains immune system homeostasis by regulating the stability and suppressive function of Tregs, as well as directly regulating the proliferation and function of conventional T cells.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | forkhead box P3, Forkhead box protein P3 41 kDa form, Forkhead box protein P3, C-terminally processed, JM2, scurfin |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
2、If thermostatic shaker is not available, will it affect the duration of my experiment and the experimental data?
Answer: If thermostatic shaker is not available, it may take 2.5 hours to complete this experiment. After adding the antibody cocktail, we suggest you incubate the plate at 37℃ for 2 hours. After adding TMB, you only need to incubate the plate at 37℃ for 15 minutes. Through comparative experiments, our R&D personnel have confirmed that you can still obtain valid experimental data, although the signal will be slightly reduced.