Mouse IFN-gamma ELISA Kit
Cat no : KE10094
Synonyms
Ifg, IFN g, IFN gamma, Ifng, interferon gamma
Validation Data Gallery
Product Information
KE10094 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Mouse IFN-gamma ELISA kit is to be used to detect and quantify protein levels of endogenous mouse IFN-gamma. The assay recognizes mouse IFN-gamma. An antibody specific for mouse IFN-gamma has been pre-coated onto the microwells. The mouse IFN-gamma protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for mouse IFN-gamma is added to detect the captured mouse IFN-gamma protein. For signal development, HRP-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse IFN-gamma ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 0.16 pg/mL |
| Range | 7.8-500 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 15978 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 93% | 80%-106% |
| Cell culture supernatants | 97% | 79%-112% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 248.9 | 5.7 | 2.3 |
| 2 | 20 | 57.1 | 1.3 | 2.3 |
| 3 | 20 | 13.5 | 0.6 | 4.3 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 254.9 | 11.5 | 4.5 |
| 2 | 24 | 60.0 | 1.5 | 2.6 |
| 3 | 24 | 14.5 | 0.5 | 3.3 |
Background Information
The IFNs were originally discovered as agents that interfere with viral replication. Initially, they were classified by the secreting cell type but are now classified into type I and type II according to receptor specificity and sequence homology. Interferon gamma (IFN-γ) is a soluble cytokine that is the only member of the type II class of interferons. It is secreted by Th1 cells, cytotoxic T cells and NK cells. The cytokine is associated with antiviral, immunoregulatory and anti-tumor properties and is a potent activator of macrophages. It plays crucial roles in pathogen clearance. Aberrant Ifng expression is associated with a number of autoinflammatory and autoimmune diseases. It has been identified in many studies as a biomarker for pleural tuberculosis (TB). Mutations in this gene are associated with aplastic anemia.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Ifg, IFN g, IFN gamma, Ifng, interferon gamma |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
Publications
| Species | Sample Type | Title |
|---|---|---|
Clin Transl Med Targeting KDM4 family epigenetically triggers antitumour immunity via enhancing tumour-intrinsic innate sensing and immunogenicity | ||
Chin Med Shaoyao Decoction reduced T lymphocyte activation by regulating of intestinal flora and 5-hydroxytryptamine metabolism in ulcerative colitis | ||
Mater Today Bio Injectable tert-butylphenylacetic acid/acrylated β-cyclodextrin-based hydrogels for co-delivery of CAR-T cells and IL-15 in solid tumor therapy. | ||
Cell Rep Med Probiotic-mediated tumor microenvironment reprogramming with protease-sensitive interleukin-15 and photothermal therapy | ||
Cancers (Basel) Induction of Immunological Antitumor Effects by the Combination of Adenovirus-Mediated Gene Transfer of B7-1 and Anti-Programmed Cell Death-1 Antibody in a Murine Squamous Cell Carcinoma Model | ||
J Colloid Interface Sci Engineered cell nanovesicle antagonists for androgen deprivation therapy of melanoma |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Flavia (Verified Customer) (04-11-2025) | The kit was easy to use and performed efficiently
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