Recombinant Mouse MGL2 protein (rFc Tag)

Species

Mouse

Purity

>90 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg2727

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Validation Data Gallery

• 

  Purity of Recombinant Mouse MGL2 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

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Product Information

• Purity: >90 %, SDS-PAGE
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Mouse MGL2 protein Ser72-Pro332 (Accession# Q8JZN1) with a rabbit IgG Fc tag at the C-terminus.
• GeneID: 216864
• Accession: Q8JZN1
• PredictedSize: 55.9 kDa
• SDS-PAGE: 50-65 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Macrophage galactose-type lectin (MGL) is a type II transmembrane glycoprotein belonging to the type II family of C-type lectin receptors (CLR). MGL is expressed on professional antigen presenting cells. In mice,there are two homologs of human MGL, MGL1(CD301a), and MGL2(CD301b). They contain the Gln–Pro–Asp (QPD) sequence in their carbohydrate recognition domain that facilitates galactose or N-acetylgalactosamine (GalNAc) recognition. Murine MGL1 was found to be highly specific for Lewis X and Lewis A structures, whereas mMGL2 recognized N-actetylgalactosamine (GalNAc) and galactose.

References:

1.Singh SK, et al. (2009) Mol Immunol. 46(6):1240-9. 
2.Denda-Nagai K, et al. (2010) J Biol Chem. 18;285(25):19193-204. 
3.Zizzari IG, et al. (2015) J Immunol Res. 2015:450695.