Mouse NGAL ELISA Kit
Cat no : KE10109
Synonyms
Lcn2, Lipocalin-2, Lipocalin 2, Neutrophil gelatinase-associated lipocalin, Oncogene 24p3
Validation Data Gallery
Product Information
KE10109 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Mouse NGAL ELISA kit is to be used to detect and quantify protein levels of endogenous mouse NGAL. The assay recognizes mouse NGAL. An antibody specific for mouse NGAL has been pre-coated onto the microwells. The mouse NGAL protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouse NGAL is added to detect the captured mouse NGAL protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse NGAL ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernates, Urine |
| Assay type | Sandwich |
| Sensitivity | 0.6 pg/mL |
| Range | 6.25-400 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 16819 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 89% | 82%-101% |
| Cell culture supernates | 94% | 85%-102% |
| Mouse urines | 92% | 83%-98% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 217.2 | 8.7 | 4.0 |
| 2 | 20 | 51.0 | 2.7 | 5.2 |
| 3 | 20 | 13.9 | 0.9 | 6.6 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 221.3 | 13.6 | 6.1 |
| 2 | 24 | 49.4 | 3.1 | 6.2 |
| 3 | 24 | 12.5 | 1.0 | 7.8 |
Background Information
Neutrophil gelatinase-associated lipocalin (NGAL) also known as Lipocalin-2 (LCN2), is a secreted glycoprotein that belongs to the lipocalin protein family. Several roles of NGAL were identified, including iron trafficking, chemotactic, and promoting cell differentiation and proliferation. NGAL was originally found in neutrophils and expressed in tissues containing renal, uterus, liver, spleen, cardiomyocytes, neurons, and immune cells. NGAL is a significant biomarker of cardiovascular diseases, ischemic stroke, acute renal injury, inflammation, and metabolic diseases.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Lcn2, Lipocalin-2, Lipocalin 2, Neutrophil gelatinase-associated lipocalin, Oncogene 24p3 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.