Mrgprx1 Polyclonal antibody

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  WB analysis using 33332-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 33332-1-AP (Mrgprx1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Mrgprx1 Polyclonal Antibody for WB, ELISA

Cat No. 33332-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

Synonyms

Mas-related G-protein coupled receptor member C11, Mas-related G-protein coupled receptor member X1, Mrgc11, Mrgprc11, Sensory neuron-specific G-protein coupled receptor 1

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Tested Applications

• Positive WB detected in: mouse liver tissue, rat heart tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

33332-1-AP targets Mrgprx1 in applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: MAS-related GPR, member X1
• Calculated Molecular Weight: 322aa, 37 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: NM_207540
• Gene Symbol: Mrgprx1
• Gene ID (NCBI): 404242
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q8CIP3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MRGPRX1 is a MAS-related G protein-coupled receptor (GPCR) primarily expressed in dorsal root ganglion (DRG) neurons and is a key receptor for itch perception. It can be activated by various endogenous peptides and exogenous ligands, triggering itch and pain signals through Gi and Gq signaling pathways. The activation of MRGPRX1 not only induces the sensation of itch in peripheral neurons but also inhibits neurotransmitter release in the spinal cord via the Gi1 pathway, alleviating chronic pain. Additionally, the structure and activation mechanism of MRGPRX1 have been elucidated, with its unique ligand-binding pocket and activation process providing a theoretical basis for the development of novel analgesic and antipruritic drugs.