ChromoTek Myc-Trap® Agarose

Myc-Trap® Agarose is an affinity reagent for IP and purification of Myc-tagged proteins. It consists of a Myc Nanobody/ VHH coupled to agarose beads.

Specificity

Myc-Tag

Applications

IP, CoIP, ChIP, RIP

Conjugate

Agarose

Cat no : yta

Synonyms

Myc tag, Myc, Myc-trap, EQKLISEEDL



Product Information

Myc-Trap® Agarose is an affinity reagent for IP and purification of Myc-tagged proteins. It consists of a Myc Nanobody/ VHH coupled to agarose beads.

ApplicationsIP, CoIP, ChIP, RIP
ClonalityMonoclonal
HostAlpaca
Affinity1x Myc-tag: Dissociation constant KD of 500 nM
2x Myc-tag: Dissociation constant KD of 0.5 nM
Specificity/TargetMyc-tag sequence motif EQKLISEEDL at the N-terminus, C-terminus, or internal site of the fusion protein. Endogenous c-myc is NOT bound.
Coupled VHHRecombinant, monoclonal anti-Myc-tag single domain antibody (sdAb) fragment
ConjugateUnconjugated
Beads~ 90 µm (cross-linked 4 % agarose beads)
Binding capacity10 µL slurry bind about 7 µg of recombinant Myc-tagged MBP
Elution buffer1x Myc peptide, 2x Myc peptide.
SDS sample buffer
0.2 M glycine pH 2.5
Storage Buffer20% EtOH
Storage ConditionShipped at ambient temperature. Upon receipt store at 4°C. Stable for one year.

Publications

ApplicationTitle
IP

Cell Cycle

Peroxisomal ACBD4 interacts with VAPB and promotes ER-peroxisome associations.

Authors - Joseph L Costello
IP

Int J Mol Sci

The ArathEULS3 Lectin Ends up in Stress Granules and Can Follow an Unconventional Route for Secretion.

Authors - Malgorzata Dubiel
IP

Cell Rep

Temporal Proteomic Analysis of Herpes Simplex Virus 1 Infection Reveals Cell-Surface Remodeling via pUL56-Mediated GOPC Degradation.

Authors - Timothy K Soh
IP

PLoS Genet

Suppression of retinal degeneration by two novel ERAD ubiquitin E3 ligases SORDD1/2 in Drosophila.

Authors - Jaiwei Xu
IP

PLoS Genet

Folliculin variants linked to Birt-Hogg-Dubé syndrome are targeted for proteasomal degradation.

Authors - Lene Clausen
IP

PLoS Genet

Makorin 1 controls embryonic patterning by alleviating Bruno1-mediated repression of oskar translation.

Authors - Annabelle Dold