ChromoTek Myc-Trap® Agarose
Myc-Trap® Agarose is an affinity reagent for IP and purification of Myc-tagged proteins. It consists of a Myc Nanobody/ VHH coupled to agarose beads.
Ready to use reagent
Specificity
Myc-Tag
Applications
IP, CoIP, ChIP, RIP
Conjugate
Agarose
Cat no : yta
Synonyms
Validation Data Gallery
Product Information
Myc-Trap® Agarose is an affinity reagent for IP and purification of Myc-tagged proteins. It consists of a Myc Nanobody/ VHH coupled to agarose beads.
| Applications | IP, CoIP, ChIP, RIP |
| Clonality | Monoclonal |
| Host | Alpaca |
| Affinity | 1x Myc-tag: Dissociation constant KD of 500 nM 2x Myc-tag: Dissociation constant KD of 0.5 nM |
| Specificity/Target | Myc-tag sequence motif EQKLISEEDL at the N-terminus, C-terminus, or internal site of the fusion protein. Endogenous c-myc is NOT bound. |
| Coupled VHH | Recombinant, monoclonal anti-Myc-tag single domain antibody (sdAb) fragment |
| Conjugate | Agarose |
| Beads | ~ 90 µm (cross-linked 4 % agarose beads) |
| Binding capacity | 10 µL slurry bind about 7 µg of recombinant Myc-tagged MBP |
| Elution buffer | 1x Myc peptide, 2x Myc peptide. SDS sample buffer 0.2 M glycine pH 2.5 |
| Storage Buffer | 20% EtOH |
| Storage Condition | Shipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. |
Documentation
| SDS |
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| SDS Myc-Trap Agarose (PDF) |
| Protocol |
|---|
| Protocol Myc-Trap Agarose (PDF) |
| Brochure |
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| Myc-Trap and anti-Myc antibody flyer (PDF) |
| Troubleshooting |
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| Troubleshooting guide immunoprecipitation (PDF) |
| Whitepapers |
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| Tips & tricks for immunoprecipitation using the Myc-Trap (PDF) |
| Myc-Trap versus conventional anti-Myc antibodies (PDF) |
Publications
| Application | Title |
|---|---|
Cell Cycle Peroxisomal ACBD4 interacts with VAPB and promotes ER-peroxisome associations. | |
Int J Mol Sci The ArathEULS3 Lectin Ends up in Stress Granules and Can Follow an Unconventional Route for Secretion. | |
Cell Rep Temporal Proteomic Analysis of Herpes Simplex Virus 1 Infection Reveals Cell-Surface Remodeling via pUL56-Mediated GOPC Degradation. | |
PLoS Genet Suppression of retinal degeneration by two novel ERAD ubiquitin E3 ligases SORDD1/2 in Drosophila. | |
PLoS Genet Folliculin variants linked to Birt-Hogg-Dubé syndrome are targeted for proteasomal degradation. | |
PLoS Genet Makorin 1 controls embryonic patterning by alleviating Bruno1-mediated repression of oskar translation. |