ChromoTek Myc-Trap® Agarose, kit

Myc-Trap® Agarose is an affinity reagent for IP and purification of Myc-tagged proteins. It consists of a Myc Nanobody/ VHH coupled to agarose beads.

Specificity

Myc-Tag

Applications

IP, CoIP, ChIP, RIP

Conjugate

Agarose

Type

Nanobody

Cat no : ytak

Synonyms

Myc tag, Myc, Myc-trap, EQKLISEEDL



Product Information

Myc-Trap® Agarose is an affinity reagent for IP and purification of Myc-tagged proteins. It consists of a Myc Nanobody/ VHH coupled to agarose beads.

DescriptionThe Myc-Trap Agarose Kit contains Myc-Trap Agarose, lysis, wash, and elution buffers for efficient immunoprecipitation of Myc-tagged proteins and their interacting factors.

• Fast, reliable & efficient one-step immunoprecipitation

• Ready-to-use

• No heavy & light antibody chains on the SDS-PAGE

• Stable under harsh washing conditions

• Elution with peptide

ApplicationsIP, CoIP, ChIP, RIP
Specificity/TargetMyc-tag sequence motif EQKLISEEDL at the N-terminus, C-terminus, or internal site of the fusion protein. Endogenous c-myc is NOT bound.
Binding capacity17.5 μg of recombinant Myc-tagged protein (~42 kDa) per 25 μL bead slurry
ConjugateAgarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads)
Elution buffer2x Myc peptide.
SDS sample buffer
0.2 M glycine pH 2.5
Wash buffer compatibility2 M NaCl, 10 mM DTT, 2 % Nonidet P40 Substitute, 1 % Triton X-100
Type Nanobody
ClassRecombinant
HostAlpaca
Affinity (KD) 1x Myc-tag: Dissociation constant KD of 500 nM
2x Myc-tag: Dissociation constant KD of 0.5 nM
Compatibility with mass spectrometryThe Myc-Trap® is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry
RRIDAB_2631369
Storage Buffer20% ethanol
Storage ConditionShipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. Do not freeze!

Kit components

ComponentDescription
Myc-Trap® Agarose20 reactions (500 µL)
Lysis bufferOptimized for cytoplasmatic proteins and mammalian cell lysis
RIPA bufferOptimized for nuclear/chromatin proteins and mammalian cell lysis
Wash buffer Removal of unwanted proteins, peptides, etc.
Dilution bufferDilution of cell lysate
Elution bufferFor acidic elution

Publications

ApplicationTitle
IP

Proc Natl Acad Sci U S A

Molecular determinants of homo- and heteromeric interactions of Junctophilin-1 at triads in adult skeletal muscle fibers.

Authors - Daniela Rossi
IP

PLoS Pathog

HEXIM1-Tat chimera inhibits HIV-1 replication.

Authors - Marie Leoz
IP

J Mol Biol

RAN Translation of the Expanded CAG Repeats in the SCA3 Disease Context.

Authors - Magdalena Jazurek-Ciesiolka
CoIP

PLoS One

The interplay of Rac1 activity, ubiquitination and GDI binding and its consequences for endothelial cell spreading.

Authors - Jisca Majolée
IP

Int J Mol Sci

Yil102c-A is a Functional Homologue of the DPMII Subunit of Dolichyl Phosphate Mannose Synthase in Saccharomyces cerevisiae.

Authors - Sebastian Piłsyk

Respir Res

The sialidase NEU3 promotes pulmonary fibrosis in mice

Authors - Darrell Pilling