ChromoTek Myc-Trap® Agarose, kit

Myc-Trap® Agarose is an affinity reagent for IP and purification of Myc-tagged proteins. It consists of a Myc Nanobody/ VHH coupled to agarose beads.

Specificity

Myc-Tag

Applications

IP, CoIP, ChIP, RIP

Conjugate

Agarose

Cat no : ytak

Synonyms

Myc tag, Myc, Myc-trap, EQKLISEEDL



Product Information

Myc-Trap® Agarose is an affinity reagent for IP and purification of Myc-tagged proteins. It consists of a Myc Nanobody/ VHH coupled to agarose beads.

ApplicationsIP, CoIP, ChIP, RIP
Clonality Monoclonal
HostAlpaca
Affinity1x Myc-tag: Dissociation constant KD of 500 nM
2x Myc-tag: Dissociation constant KD of 0.5 nM
Specificity/TargetMyc-tag sequence motif EQKLISEEDL at the N-terminus, C-terminus, or internal site of the fusion protein. Endogenous c-myc is NOT bound.
Coupled VHHRecombinant, monoclonal anti-Myc-tag single domain antibody (sdAb) fragment
ConjugateAgarose
Beads~ 90 µm (cross-linked 4 % agarose beads)
Binding capacity10 µL slurry bind about 7 µg of recombinant Myc-tagged MBP
Elution buffer1x Myc peptide, 2x Myc peptide.
SDS sample buffer
0.2 M glycine pH 2.5
Storage Buffer20% EtOH
Storage ConditionShipped at ambient temperature. Upon receipt store at 4°C. Stable for one year.

Kit components

ComponentDescription
Myc-Trap® Agarose 20 reactions (500 µL)
Lysis buffer Optimized for cytoplasmatic proteins and mammalian cell lysis
RIPA buffer Optimized for nuclear/chromatin proteins and mammalian cell lysis
Wash buffer Removal of unwanted proteins, peptides, etc.
Dilution buffer Dilution of cell lysate
Elution buffer For acidic elution

Publications

ApplicationTitle
IP

PLoS Pathog

HEXIM1-Tat chimera inhibits HIV-1 replication.

Authors - Marie Leoz
IP

Proc Natl Acad Sci U S A

Molecular determinants of homo- and heteromeric interactions of Junctophilin-1 at triads in adult skeletal muscle fibers.

Authors - Daniela Rossi