ChromoTek Myc-Trap® Agarose, kit

Myc-Trap® Agarose is an affinity reagent for IP and purification of Myc-tagged proteins. It consists of a Myc Nanobody/ VHH coupled to agarose beads.

Specificity

Myc-Tag

Applications

IP, CoIP, ChIP, RIP

Conjugate

Agarose

Cat no : ytak

Synonyms

Myc tag, Myc, Myc-trap, EQKLISEEDL



Product Information

Myc-Trap® Agarose is an affinity reagent for IP and purification of Myc-tagged proteins. It consists of a Myc Nanobody/ VHH coupled to agarose beads.

ApplicationsIP, CoIP, ChIP, RIP
Clonality VHH
HostAlpaca
Affinity1x Myc-tag: Dissociation constant KD of 500 nM
2x Myc-tag: Dissociation constant KD of 0.5 nM
Specificity/TargetMyc-tag sequence motif EQKLISEEDL at the N-terminus, C-terminus, or internal site of the fusion protein. Endogenous c-myc is NOT bound.
Coupled VHHRecombinant, monoclonal anti-Myc-tag single domain antibody (sdAb) fragment
ConjugateAgarose
Beads~ 90 µm (cross-linked 4 % agarose beads)
Binding capacity10 µL slurry bind about 7 µg of recombinant Myc-tagged MBP
Elution buffer1x Myc peptide, 2x Myc peptide.
SDS sample buffer
0.2 M glycine pH 2.5
Storage Buffer20% EtOH
Storage ConditionShipped at ambient temperature. Upon receipt store at 4°C. Stable for one year.

Kit components

ComponentDescription
Myc-Trap® Agarose20 reactions (500 µL)
Lysis bufferOptimized for cytoplasmatic proteins and mammalian cell lysis
RIPA bufferOptimized for nuclear/chromatin proteins and mammalian cell lysis
Wash buffer Removal of unwanted proteins, peptides, etc.
Dilution bufferDilution of cell lysate
Elution bufferFor acidic elution

Publications

ApplicationTitle
IP

Int J Mol Sci

Yil102c-A is a Functional Homologue of the DPMII Subunit of Dolichyl Phosphate Mannose Synthase in Saccharomyces cerevisiae.

Authors - Sebastian Piłsyk
IP

PLoS Pathog

HEXIM1-Tat chimera inhibits HIV-1 replication.

Authors - Marie Leoz
IP

Proc Natl Acad Sci U S A

Molecular determinants of homo- and heteromeric interactions of Junctophilin-1 at triads in adult skeletal muscle fibers.

Authors - Daniela Rossi