ChromoTek Myc-Trap® Magnetic Agarose

The ChromoTek Myc-Trap® Magnetic Agarose is an affinity resin for IP of Myc-fusion proteins. It consists of a Myc Nanobody/VHH coupled to magnetic agarose beads.

Specificity

Myc-Tag

Applications

IP, CoIP, ChIP, RIP

Conjugate

Magnetic Agarose

Publications

41

Cat no : ytma

Synonyms

Myc tag, Myc, Myc-trap, EQKLISEEDL

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Validation Data Gallery

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  Myc-Trap Magnetic Agarose for pull-down of Myc-tagged proteins.

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Product Information

The ChromoTek Myc-Trap® Magnetic Agarose is an affinity resin for IP of Myc-fusion proteins. It consists of a Myc Nanobody/VHH coupled to magnetic agarose beads.

• Applications: IP, CoIP, ChIP, RIP
• Clonality: Monoclonal
• Host: Alpaca
• Affinity: 1x Myc-tag: Dissociation constant KD of 500 nM; 2x Myc-tag: Dissociation constant KD of 0.5 nM
• Specificity/Target: Myc-tag sequence motif EQKLISEEDL at the N-terminus, C-terminus, or internal site of the fusion protein. Endogenous c-myc is NOT bound.
• Coupled VHH: recombinant, monoclonal anti-Myc-tag single domain antibody (sdAb) fragment
• Conjugate: Magnetic Agarose
• Beads: ~ 40 µm
• Binding capacity: 10 µL slurry bind ~ 7 µg of recombinant Myc-tagged MBP
• Elution buffer: 1x Myc peptide, 2x Myc peptide.; SDS sample buffer; 0.2 M glycine pH 2.5
• Storage Buffer: 20% EtOH
• Storage Condition: Shipped at ambient temperature. Upon receipt store at 4°C. Stable for one year.

Publications

Application	Title
CoIP	Cell Rep; The Influenza A Virus Endoribonuclease PA-X Usurps Host mRNA Processing Machinery to Limit Host Gene Expression.; Authors - Lea Gaucherand
IP	Cell Rep; Vaccinia Virus Ankyrin-Repeat/F-Box Protein Targets Interferon-Induced IFITs for Proteasomal Degradation.; Authors - Ruikang Liu
IP	Cell Rep; mTORC2 Assembly Is Regulated by USP9X-Mediated Deubiquitination of RICTOR.; Authors - Lidia Wrobel
IP	Cell Rep; Snd3 controls nucleus-vacuole junctions in response to glucose signaling.; Authors - Sergi Tosal-Castano
IP	Protein Expr Purif; Enhancement of cell proliferation and motility of mammalian cells grown in co-culture with Pichia pastoris expressing recombinant human FGF-2.; Authors - Henry Hieu M Le
IP	Curr Microbiol; Characterization of a Phosphatidylserine Synthase of Vibrio parahaemolyticus.; Authors - Ru-Yin Huang