ChromoTek Myc-Trap® Magnetic Agarose
The ChromoTek Myc-Trap® Magnetic Agarose is an affinity resin for IP of Myc-fusion proteins. It consists of a Myc Nanobody/VHH coupled to magnetic agarose beads.
Ready to use reagent
Specificity
Myc-Tag
Applications
IP, CoIP, ChIP, RIP
Conjugate
Magnetic Agarose
Cat no : ytma
Synonyms
Validation Data Gallery
Product Information
The ChromoTek Myc-Trap® Magnetic Agarose is an affinity resin for IP of Myc-fusion proteins. It consists of a Myc Nanobody/VHH coupled to magnetic agarose beads.
Applications | IP, CoIP, ChIP, RIP |
Clonality | Monoclonal |
Host | Alpaca |
Affinity | 1x Myc-tag: Dissociation constant KD of 500 nM 2x Myc-tag: Dissociation constant KD of 0.5 nM |
Specificity/Target | Myc-tag sequence motif EQKLISEEDL at the N-terminus, C-terminus, or internal site of the fusion protein. Endogenous c-myc is NOT bound. |
Coupled VHH | recombinant, monoclonal anti-Myc-tag single domain antibody (sdAb) fragment |
Conjugate | Magnetic Agarose |
Beads | ~ 40 µm |
Binding capacity | 10 µL slurry bind ~ 7 µg of recombinant Myc-tagged MBP |
Elution buffer | 1x Myc peptide, 2x Myc peptide. SDS sample buffer 0.2 M glycine pH 2.5 |
Storage Buffer | 20% EtOH |
Storage Condition | Shipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. |
Documentation
SDS |
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SDS Myc-Trap Magnetic Agarose (PDF) |
Protocol |
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Protocol Myc-Trap Magnetic Agarose (PDF) |
Brochure |
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Myc-Trap and anti-Myc antibody flyer (PDF) |
Troubleshooting |
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Troubleshooting guide immunoprecipitation (PDF) |
Whitepapers |
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Tips & tricks for immunoprecipitation using the Myc-Trap (PDF) |
Myc-Trap versus conventional anti-Myc antibodies (PDF) |
Publications
Application | Title |
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Cell Rep The Influenza A Virus Endoribonuclease PA-X Usurps Host mRNA Processing Machinery to Limit Host Gene Expression. | |
Cell Rep Vaccinia Virus Ankyrin-Repeat/F-Box Protein Targets Interferon-Induced IFITs for Proteasomal Degradation. | |
Curr Microbiol Characterization of a Phosphatidylserine Synthase of Vibrio parahaemolyticus. | |
Sci Rep Chemically monoubiquitinated PEX5 binds to the components of the peroxisomal docking and export machinery. | |
J Cell Sci Selective transport of neurotransmitters and modulators by distinct volume-regulated LRRC8 anion channels. |