ChromoTek Myc-Trap® Magnetic Agarose

The ChromoTek Myc-Trap® Magnetic Agarose is an affinity resin for IP of Myc-fusion proteins. It consists of a Myc Nanobody/VHH coupled to magnetic agarose beads.

Ready to use reagent

Specificity

Myc-Tag

Applications

IP, CoIP, ChIP, RIP

Conjugate

Magnetic Agarose

Cat no : ytma

Synonyms

Myc tag, Myc, Myc-trap, EQKLISEEDL



Product Information

The ChromoTek Myc-Trap® Magnetic Agarose is an affinity resin for IP of Myc-fusion proteins. It consists of a Myc Nanobody/VHH coupled to magnetic agarose beads.

ApplicationsIP, CoIP, ChIP, RIP
Clonality Monoclonal
Host Alpaca
Affinity1x Myc-tag: Dissociation constant KD of 500 nM
2x Myc-tag: Dissociation constant KD of 0.5 nM
Specificity/TargetMyc-tag sequence motif EQKLISEEDL at the N-terminus, C-terminus, or internal site of the fusion protein. Endogenous c-myc is NOT bound.
Coupled VHHrecombinant, monoclonal anti-Myc-tag single domain antibody (sdAb) fragment
ConjugateMagnetic Agarose
Beads~ 40 µm
Binding capacity10 µL slurry bind ~ 7 µg of recombinant Myc-tagged MBP
Elution buffer1x Myc peptide, 2x Myc peptide.
SDS sample buffer
0.2 M glycine pH 2.5
Storage Buffer20% EtOH
Storage ConditionShipped at ambient temperature. Upon receipt store at 4°C. Stable for one year.

Publications

ApplicationTitle
CoIP

Cell Rep

The Influenza A Virus Endoribonuclease PA-X Usurps Host mRNA Processing Machinery to Limit Host Gene Expression.

Authors - Lea Gaucherand
IP-MS

Cell Rep

Vaccinia Virus Ankyrin-Repeat/F-Box Protein Targets Interferon-Induced IFITs for Proteasomal Degradation.

Authors - Ruikang Liu
IP

Cell Rep

mTORC2 Assembly Is Regulated by USP9X-Mediated Deubiquitination of RICTOR.

Authors - Lidia Wrobel
IP

Curr Microbiol

Characterization of a Phosphatidylserine Synthase of Vibrio parahaemolyticus.

Authors - Ru-Yin Huang
IP

Sci Rep

Chemically monoubiquitinated PEX5 binds to the components of the peroxisomal docking and export machinery.

Authors - Vera Hagmann
IP

J Cell Sci

Selective transport of neurotransmitters and modulators by distinct volume-regulated LRRC8 anion channels.

Authors - Darius Lutter