ChromoTek Myc-Trap® Magnetic Agarose
The ChromoTek Myc-Trap® Magnetic Agarose is an affinity resin for IP of Myc-fusion proteins. It consists of a Myc Nanobody/VHH coupled to magnetic agarose beads.
Specificity
Myc-Tag
Applications
IP, CoIP, ChIP, RIP
Conjugate
Magnetic Agarose
Cat no : ytma
Synonyms
Validation Data Gallery
Product Information
The ChromoTek Myc-Trap® Magnetic Agarose is an affinity resin for IP of Myc-fusion proteins. It consists of a Myc Nanobody/VHH coupled to magnetic agarose beads.
| Applications | IP, CoIP, ChIP, RIP |
| Clonality | Monoclonal |
| Host | Alpaca |
| Affinity | 1x Myc-tag: Dissociation constant KD of 500 nM 2x Myc-tag: Dissociation constant KD of 0.5 nM |
| Specificity/Target | Myc-tag sequence motif EQKLISEEDL at the N-terminus, C-terminus, or internal site of the fusion protein. Endogenous c-myc is NOT bound. |
| Coupled VHH | recombinant, monoclonal anti-Myc-tag single domain antibody (sdAb) fragment |
| Conjugate | Magnetic Agarose |
| Beads | ~ 40 µm |
| Binding capacity | 10 µL slurry bind ~ 7 µg of recombinant Myc-tagged MBP |
| Elution buffer | 1x Myc peptide, 2x Myc peptide. SDS sample buffer 0.2 M glycine pH 2.5 |
| Storage Buffer | 20% EtOH |
| Storage Condition | Shipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. |
Documentation
| SDS |
|---|
| SDS Myc-Trap Magnetic Agarose (PDF) |
| Protocol |
|---|
| Protocol Myc-Trap Magnetic Agarose (PDF) |
| Whitepapers |
|---|
| Myc-Trap versus conventional anti-Myc antibodies (PDF) |
| Tips & tricks for immunoprecipitation using the Myc-Trap (PDF) |
| Brochure |
|---|
| Myc-Trap and anti-Myc antibody flyer (PDF) |
| Troubleshooting |
|---|
| Troubleshooting guide immunoprecipitation (PDF) |
Publications
| Application | Title |
|---|---|
Cell Rep The Influenza A Virus Endoribonuclease PA-X Usurps Host mRNA Processing Machinery to Limit Host Gene Expression. | |
Cell Rep Vaccinia Virus Ankyrin-Repeat/F-Box Protein Targets Interferon-Induced IFITs for Proteasomal Degradation. | |
JCI Insight RB expression confers sensitivity to CDK4/6 inhibitor-mediated radiosensitization across breast cancer subtypes. | |
Protein Expr Purif Enhancement of cell proliferation and motility of mammalian cells grown in co-culture with Pichia pastoris expressing recombinant human FGF-2. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Amy (Verified Customer) (06-10-2022) | Clean and efficient precipitation of Myc-tagged bait proteins, HEK293T cells transfected with Myc-tagged proteins and lysed in RIPA buffer prior to precipitation with Myc-Trap magnetic agarose beads.
|