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N-cadherin Monoclonal antibody

N-cadherin Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1


human, pig, mouse, rat, rabbit







Cat no : 66219-1-Ig


Cadherin 2, CD325, CDH2, CDHN, CDw325, N cadherin, NCAD, N-cadherin, Neural cadherin

Tested Applications

Positive WB detected inrabbit brain tissue, pig brain tissue, human heart tissue, MKN-45 cells, HEK-293 cells, PC-3 cells, mouse brain tissue, rat brain tissue, C2C12 cells, C6 cells, Saos-2 cells, SGC-7901 cells, ROS1728 cells, NIH/3T3 cells
Positive IHC detected inmouse heart tissue, mouse brain tissue, human malignant melanoma tissue, human ovary tumor tissue, rat heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse heart tissue

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:7500-1:30000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66219-1-Ig targets N-cadherin in WB, IF, IHC, ELISA applications and shows reactivity with human, pig, mouse, rat, rabbit samples.

Tested Reactivity human, pig, mouse, rat, rabbit
Cited Reactivityhuman, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen N-cadherin fusion protein Ag4996
Full Name cadherin 2, type 1, N-cadherin (neuronal)
Calculated Molecular Weight 906 aa, 100 kDa
Observed Molecular Weight 130 kDa
GenBank Accession NumberBC036470
Gene Symbol CDH2
Gene ID (NCBI) 1000
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. N-cadherin (neural cadherin), also known as CDH2 (cadherin 2), is a 130-kDa transmembrane protein and a classical member of the cadherin superfamily which also include E-, P-, R-, and B-cadherins. Expression of N-cadherin has been reported on various cell types including neurons, endothelial cells and cardiac myocytes (PMID: 11282032; 9508779; 8125202). N-cadherin has functions in early brain morphogenesis, synaptogenesis and synaptic plasticity (PMID: 23321619). The N-cadherin ectodomain can be cleaved, leading to the generation of a 90 kD N-terminal fragment (PMID: 16998833; 17028923).


Product Specific Protocols
WB protocol for N-cadherin antibody 66219-1-IgDownload protocol
IHC protocol for N-cadherin antibody 66219-1-IgDownload protocol
IF protocol for N-cadherin antibody 66219-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Davide (Verified Customer) (06-13-2023)

The antibody didn't produce any signal in 3d primary human cholangiocyte organoids. I would recommend either to try other antibody concentration or to try another Ab. It was tried on a cell condition which was recently reported to stimulate the CDH2 protein expression. However it should also be present in control condition at low levels.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Cholangiocyte organoids
N-cadherin Antibody Immunofluorescence validation (1:100 dilution) in Cholangiocyte organoids (Cat no:66219-1-Ig)

Udesh (Verified Customer) (10-13-2022)

Used the Ab at 1:2000 dilution in 5% NFDM and observed a band at 140 kD. Blocked in 5% NFDM for 1H at RT.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: U2OS Osteosarcoma cells
N-cadherin Antibody Western Blot validation (1:2000 dilution) in U2OS Osteosarcoma cells (Cat no:66219-1-Ig)