Validation Data Gallery
|Positive WB detected in||MKN-45 cells, HEK-293 cells, PC-3 cells, human heart tissue, pig brain tissue, rat brain tissue, SGC-7901 cells, ROS1728 cells, NIH/3T3 cells, C6 cells|
|Positive IHC detected in||mouse heart tissue, mouse brain tissue, rat heart tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||mouse heart tissue|
|Western Blot (WB)||WB : 1:2000-1:10000|
|Immunohistochemistry (IHC)||IHC : 1:7500-1:30000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
66219-1-Ig targets N-cadherin in WB, IHC, IF applications and shows reactivity with human, pig, mouse, rat samples.
|Tested Reactivity||human, pig, mouse, rat|
|Cited Reactivity||human, mouse, pig, rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||N-cadherin fusion protein Ag4996|
|Full Name||cadherin 2, type 1, N-cadherin (neuronal)|
|Calculated molecular weight||906 aa, 100 kDa|
|Observed molecular weight||130 kDa|
|GenBank accession number||BC036470|
|Gene ID (NCBI)||1000|
|Purification Method||Protein G purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. N-cadherin (neural cadherin), also known as CDH2 (cadherin 2), is a 130-kDa transmembrane protein and a classical member of the cadherin superfamily which also include E-, P-, R-, and B-cadherins. Expression of N-cadherin has been reported on various cell types including neurons, endothelial cells and cardiac myocytes (PMID: 11282032; 9508779; 8125202). N-cadherin has functions in early brain morphogenesis, synaptogenesis and synaptic plasticity (PMID: 23321619).
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