Tested Applications
| Positive WB detected in | HEK-293 cells |
| Positive IHC detected in | mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16211-1-AP targets N6AMT1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9204 Product name: Recombinant human N6AMT1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-186 aa of BC011554 Sequence: MAGENFATPFHGHVGRGAFSDVYEPAEDTFLLLDALEAAAAELAGVEICLEVGSGSGVVSAFLASMIGPQALYMCTDINPEAAACTLETARCNKVHIQPVITDLVGSHGIEAAWAGGRNGREVMDRFFPLVPDLLSPRGLFYLVTIKENNPEEILKIMKTKGLQGTTALSRQAGQETLSVLKFTKS Predict reactive species |
| Full Name | N-6 adenine-specific DNA methyltransferase 1 (putative) |
| Calculated Molecular Weight | 186 aa, 20 kDa |
| Observed Molecular Weight | 23 kDa |
| GenBank Accession Number | BC011554 |
| Gene Symbol | N6AMT1 |
| Gene ID (NCBI) | 29104 |
| RRID | AB_2147186 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y5N5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for N6AMT1 antibody 16211-1-AP | Download protocol |
| WB protocol for N6AMT1 antibody 16211-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The N6AMT1 Polyclonal antibody (Cat# 16211-1-AP) has 6 citations published in Experimental Hematology & Oncology, Cell Death & Disease, Clinical and Translational Medicine, Cellular and Molecular Life Sciences, Antibodies (Basel), and Microbiology and Immunology. Research fields span DNA epigenetic modification, colorectal cancer, atherosclerosis, ferroptosis in gastrointestinal stromal tumors, hippocampal neurogenesis, antibody specificity, and virology.
| Species | Application | Title |
|---|---|---|
Exp Hematol Oncol Extracellular matrix stiffness reduces DNA 6 ma level to facilitate colorectal cancer progression via disrupting P53 binding to CDKN1A promoter | ||
Cell Death Dis Association of N6-methyladenine DNA with plaque progression in atherosclerosis via myocardial infarction-associated transcripts. | ||
Clin Transl Med β-elemene promotes ferroptosis to improve the sensitivity of imatinib in gastrointestinal stromal tumours by targeting N6AMT1 | ||
Cell Mol Life Sci The methyltransferase N6amt1 regulates hippocampal neurogenesis through 6 mA modification of Txnrd3 DNA.
| ||
Antibodies (Basel) Cross-Reactivity of N6AMT1 Antibodies with Aurora Kinase A: An Example of Antibody-Specific Non-Specificity | ||
Microbiol Immunol BUD23-TRMT112 interacts with the L protein of Borna disease virus and mediates the chromosomal tethering of viral ribonucleoproteins. |
