NBCn1 Polyclonal antibody
NBCn1 Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human
Applications
WB, ELISA
Conjugate
Unconjugated
Cat no : 29442-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | BGC-823 cells, HEK-293 cells, HeLa cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:1000-1:5000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
29442-1-AP targets NBCn1 in WB, ELISA applications and shows reactivity with Human samples.
Tested Reactivity | Human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | NBCn1 fusion protein Ag30545 |
Full Name | solute carrier family 4, sodium bicarbonate cotransporter, member 7 |
Calculated Molecular Weight | 136 kDa |
Observed Molecular Weight | 136 kDa |
GenBank Accession Number | NM_003615 |
Gene Symbol | SLC4A7 |
Gene ID (NCBI) | 9497 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SLC4A7 also known as NBCn1, NBC3, NBC2, is an electroneutral Na(+)/HCO(3)(-) cotransporter (PMID: 14736710). SLC4A7 mediates the coupled movement of sodium and bicarbonate ions across the plasma membrane. SLC4A7 plays a key role in macrophage acidification, mediating bicarbonate import into the cytoplasm which is crucial for net acid extrusion and maintenance of cytoplasmic pH during phagocytosis (PubMed:29779931). SLC4A7 is expressed in testis, spleen, and retina. SLC4A7 may be a major regulator of extracellular pH of retina where light stimulation produces an extracellular alkalization and may contribute as a solute transporter to the prevention of retinal detachment(PMID: 9610397).
Protocols
Product Specific Protocols | |
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WB protocol for NBCn1 antibody 29442-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |