NBN / NBS1 Polyclonal antibody, PBS Only

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• NBN / NBS1 Polyclonal antibody, PBS Only (55025-1-PBS)

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  WB analysis using 55025-1-AP (same clone as 55025-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 55025-1-AP (NBS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55025-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 55025-1-AP (same clone as 55025-1-PBS)

  WB result of NBN / NBS1 antibody (55025-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NBN / NBS1 transfected HeLa cells. This data was developed using the same antibody clone with 55025-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 55025-1-AP (same clone as 55025-1-PBS)

  IP result of anti-NBN / NBS1 (IP:55025-1-AP, 4ug; Detection:55025-1-AP 1:3000) with HeLa cells lysate 1320 ug. This data was developed using the same antibody clone with 55025-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach using 55025-1-AP (same clone as 55025-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 55025-1-AP (NBN / NBS1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55025-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach using 55025-1-AP (same clone as 55025-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 55025-1-AP (NBN / NBS1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55025-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 55025-1-AP (same clone as 55025-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using NBN / NBS1 antibody (55025-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 55025-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 55025-1-AP (same clone as 55025-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using 55025-1-AP (NBS1 antibody) at dilution of 1:200 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 55025-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

NBN / NBS1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 55025-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

NBN, AT V1, AT V2, ATV, FLJ10155

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Product Information

55025-1-PBS targets NBN / NBS1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: nibrin
• Calculated Molecular Weight: 85 kDa
• Observed Molecular Weight: 90-95 kDa
• GenBank Accession Number: NM_002485
• Gene Symbol: NBN
• Gene ID (NCBI): 4683
• RRID: AB_10858928
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O60934
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

NBN, also named as NBS, NBS1 and P95, is a component of the MRE11/RAD50/NBN (MRN complex) which plays a critical role in the cellular response to DNA damage and the maintenance of chromosome integrity. The complex is involved in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity, cell cycle checkpoint control and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. NBN modulate the DNA damage signal sensing by recruiting PI3/PI4-kinase family members ATM, ATR, and probably DNA-PKcs to the DNA damage sites and activating their functions. NBN also functions in telomere length maintenance by generating the 3' overhang which serves as a primer for telomerase dependent telomere elongation. NBN is a major player in the control of intra-S-phase checkpoint and there is some evidence that NBN is involved in G1 and G2 checkpoints. Defects in NBN are the cause of Nijmegen breakage syndrome (NBS). Defects in NBN are a cause of genetic susceptibility to breast cancer (BC). Defects in NBN may be associated with aplastic anemia. Defects in NBN might play a role in the pathogenesis of childhood acute lymphoblastic leukemia (ALL). The antibody is specific to NBN. The full-length NBN protein, with an apparent molecular weight of 95 kDa and the two protein fragments of 26 and 70 kDa arising from the c.657_661del5 (p.K219fsX19) mutation, and the 80 kDa protein found in patient RR with the mutation c.742_743insGG leading to excision of exons 6 and 7 from the NBN mRNA are shown. (PMID: 26265251) The predicted molecular weight of NBN protein (p95) is 85kDa, actually detection result is about 95kDa(PMID: 23762398).