NBPF1 Polyclonal antibody

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  IP experiment of HeLa using 13242-1-AP

  IP result of anti-NBPF1 (IP:13242-1-AP, 4ug; Detection:13242-1-AP 1:300) with HeLa cells lysate 1760 ug.

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  IHC staining of human liver using 13242-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13242-1-AP (NBPF1 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of Jurkat using 13242-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using NBPF1 antibody (13242-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

NBPF1 Polyclonal Antibody for IHC, IF/ICC, IP, ELISA

Cat No. 13242-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, IP, ELISA

Synonyms

Neuroblastoma breakpoint family member 1, KIAA1693

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Tested Applications

• Positive IP detected in: HeLa cells
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: Jurkat cells

Recommended dilution

• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13242-1-AP targets NBPF1 in IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NBPF1 fusion protein Ag4096
• Full Name: neuroblastoma breakpoint family, member 1
• Calculated Molecular Weight: 1213 aa, 139 kDa
• Observed Molecular Weight: 139 kDa
• GenBank Accession Number: BC034418
• Gene Symbol: NBPF1
• Gene ID (NCBI): 55672
• RRID: AB_3669156
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q3BBV0
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NBPF1 (Neuroblastoma Breakpoint Family, member 1) was originally identified in a neuroblastoma patient on the basis of its disruption by a chromosomal translocation t(1;17)