NCX1 Polyclonal antibody, PBS Only

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  WB analysis using 28447-1-AP (same clone as 28447-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 28447-1-AP (NCX1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28447-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 28447-1-AP (same clone as 28447-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 28447-1-AP (NCX1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28447-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 28447-1-AP (same clone as 28447-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 28447-1-AP (NCX1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28447-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 28447-1-AP (same clone as 28447-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 28447-1-AP (NCX1 antibody) at dilution of 1:1600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28447-1-PBS in a different storage buffer formulation.

NCX1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 28447-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Na(+)/Ca(2+)-exchange protein 1, SLC8A1, Solute carrier family 8 member 1

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Product Information

28447-1-PBS targets NCX1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NCX1 fusion protein Ag28952
• Full Name: solute carrier family 8 (sodium/calcium exchanger), member 1
• Calculated Molecular Weight: 109 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: NM_021097
• Gene Symbol: NCX1
• Gene ID (NCBI): 6546
• RRID: AB_2918167
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P32418
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

SLC8A1, also known as NCX1, belongs to the SLC (Solute Carrier) family and the CaCA (Calcium/Cation Antiporter) superfamily. NCX1 is a bidirectional ion exchanger that can exchange sodium and calcium ions between the inside and outside of cells in a 1:3 ratio (1 Ca2+ ion and 3 Na+ ions). Under physiological conditions, NCX1 mainly transports Ca2+ from the cell interior to the exterior through the forward transport mode to maintain the low calcium level inside the cell. However, under certain special conditions, such as ischemia-reperfusion injury and stroke, the reverse transport mode of NCX1 is activated, leading to the influx of Ca2+ into the cell, which may cause cell damage (PMID: 21289289, 30686898).