ND4 Polyclonal antibody

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• ND4 Polyclonal antibody (26736-1-AP)

• 

  WB analysis using 26736-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26736-1-AP (ND4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 26736-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 26736-1-AP (ND4 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ND4 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 26736-1-AP

Publications(7)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, ELISA

Synonyms

MT-ND4, MT ND4, MTND4, NADH4

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Tested Applications

• Positive WB detected in: mouse kidney tissue
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 7 publications below

Product Information

26736-1-AP targets ND4 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: NADH dehydrogenase, subunit 4 (complex I)
• Calculated Molecular Weight: 52 kDa
• Observed Molecular Weight: 43-52 kDa
• GenBank Accession Number: YP_003024035
• Gene Symbol: ND4
• Gene ID (NCBI): 4538
• RRID: AB_3085898
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P03905
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NADH dehydrogenase 4 (ND4) is one of the core subunits of mitochondrial respiratory chain Complex I and is essential for cellular energy production. Complex I is the first step involved in cellular aerobic respiration (i.e., oxidative phosphorylation), responsible for catalyzing the transfer of electrons from NADH to coenzyme Q10 (ubiquinone), thereby initiating the entire electron transport chain.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Aging (Albany NY); Mitochondrial dysfunction following repeated administration of alprazolam causes attenuation of hippocampus-dependent memory consolidation in mice; Authors - Siqing Zhu
mouse	WB	Sci Rep; Oxidative stress imbalance and cellular damage mediated by the ND4 G11778A mutation.; Authors - Lijun Fang
human	WB	Mol Med Rep; Direct effects of the small molecule PD‑L1 inhibitor BMS‑202 on A375 melanoma cells: Anti‑tumor activity accompanied by increased mitochondrial function; Authors - Nieng Zhang
human	WB	Nat Commun; FUS unveiled in mitochondrial DNA repair and targeted ligase-1 expression rescues repair-defects in FUS-linked motor neuron disease; Authors - Manohar Kodavati
mouse	WB	Cell Metab; Aging-induced tRNAGlu-derived fragment impairs glutamate biosynthesis by targeting mitochondrial translation-dependent cristae organization; Authors - Dingfeng Li
human	WB	Free Radic Biol Med; Oxidative stress-induced decreased expression of FABP5 leads to mitochondrial damage and survival disorder of decidual stromal cells in women with recurrent spontaneous abortion; Authors - Dong Li