ND4 Polyclonal antibody

ND4 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 26736-1-AP

Synonyms

MT ND4, MTND4, MT-ND4, NADH dehydrogenase subunit 4, NADH4, ND4

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Validation Data Gallery

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  WB analysis using 26736-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26736-1-AP (ND4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse kidney tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

26736-1-AP targets ND4 in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: NADH dehydrogenase, subunit 4 (complex I)
• Calculated Molecular Weight: 52 kDa
• Observed Molecular Weight: 43-52 kDa
• GenBank Accession Number: YP_003024035
• Gene Symbol: ND4
• Gene ID (NCBI): 4538
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Aging (Albany NY); Mitochondrial dysfunction following repeated administration of alprazolam causes attenuation of hippocampus-dependent memory consolidation in mice; Authors - Siqing Zhu
human	WB	Nat Commun; FUS unveiled in mitochondrial DNA repair and targeted ligase-1 expression rescues repair-defects in FUS-linked motor neuron disease; Authors - Manohar Kodavati