NDUFC2 Polyclonal antibody

NDUFC2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 15573-1-AP

Synonyms

B14.5b, CI B14.5b, Complex I B14.5b, HLC 1, NADHDH2, NDUFC2

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Validation Data Gallery

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  WB analysis using 15573-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15573-1-AP (NDUFC2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 15573-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 15573-1-AP (NDUFC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 15573-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 15573-1-AP (NDUFC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 15573-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15573-1-AP (NDUFC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 15573-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 15573-1-AP (NDUFC2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 15573-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15573-1-AP (NDUFC2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 15573-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15573-1-AP (NDUFC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 15573-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15573-1-AP (NDUFC2 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 15573-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15573-1-AP (NDUFC2 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human hepatocirrhosis using 15573-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 15573-1-AP (NDUFC2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human hepatocirrhosis using 15573-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 15573-1-AP (NDUFC2 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: A549 cells, human liver tissue, human kidney tissue, Hela cells, HepG2 cells
• Positive IHC detected in: human kidney tissue, human hepatocirrhosis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

The immunogen of 15573-1-AP is NDUFC2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NDUFC2 fusion protein Ag7919
• Full Name: NADH dehydrogenase (ubiquinone) 1, subcomplex unknown, 2, 14.5kDa
• Calculated Molecular Weight: 14 kDa
• Observed Molecular Weight: 14 kDa
• GenBank Accession Number: BC007323
• Gene Symbol: NDUFC2
• Gene ID (NCBI): 4718
• RRID: AB_10666733
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Nat Commun; Intellectual disability-associated factor Zbtb11 cooperates with NRF-2/GABP to control mitochondrial function.; Authors - Brooke C Wilson
pig	WB	Sci Rep; Proteomic analysis indicates that mitochondrial energy metabolism in skeletal muscle tissue is negatively correlated with feed efficiency in pigs.; Authors - Liangliang Fu
human	WB	Cell Rep; A membrane arm of mitochondrial complex I sufficient to promote respirasome formation.; Authors - Hezhi Fang