NDUFV3 Polyclonal antibody

NDUFV3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 13430-1-AP

Synonyms

Renal carcinoma antigen NY-REN-4, NADH-ubiquinone oxidoreductase 9 kDa subunit, Complex I-9kD, Complex I 9kD, CI-9kD

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Validation Data Gallery

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  WB analysis of HepG2 using 13430-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13430-1-AP (NDUFV3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 13430-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 13430-1-AP (NDUFV3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 13430-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 13430-1-AP (NDUFV3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 13430-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13430-1-AP (NDUFV3 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human placenta using 13430-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13430-1-AP (NDUFV3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 13430-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13430-1-AP (NDUFV3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 13430-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13430-1-AP (NDUFV3 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of HepG2 using 13430-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 13430-1-AP (NDUFV3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HepG2 cells, human heart tissue, human liver tissue
• Positive IHC detected in: human placenta tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below

Product Information

13430-1-AP targets NDUFV3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NDUFV3 fusion protein Ag4234
• Full Name: NADH dehydrogenase (ubiquinone) flavoprotein 3, 10kDa
• Calculated Molecular Weight: 108 aa, 12 kDa
• Observed Molecular Weight: 10 kDa
• GenBank Accession Number: BC033766
• Gene Symbol: NDUFV3
• Gene ID (NCBI): 4731
• RRID: AB_2282462
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NDUFV3(NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial) is also named as CI-9kD,Renal carcinoma antigen NY-REN-4 and belongs to the complex I NDUFV3 subunit family.It is an accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis.

Publications

Species	Application	Title
human	WB	J Proteome Res; Quantitative proteomics reveals oxygen-dependent changes in neuronal mitochondria affecting function and sensitivity to rotenone.; Authors - Villeneuve Lance L
human	WB	J Pharmacol Exp Ther; Metabolic Mechanisms and a Rational Combinational Application of Carboxyamidotriazole in Fighting Pancreatic Cancer Progression after Chemotherapy.; Authors - Rui Ju
human	WB	FEBS Lett; A novel isoform of the human mitochondrial complex I subunit NDUFV3.; Authors - Marris G Dibley
human	WB	F1000Res; Androgen-dependent alternative mRNA isoform expression in prostate cancer cells.; Authors - Jennifer Munkley
mouse	WB	Nat Struct Mol Biol; SCAF1 drives the compositional diversity of mammalian respirasomes; Authors - Irene Vercellino