NEDD8 Polyclonal antibody

NEDD8 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, monkey

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 16777-1-AP

Synonyms

cul nedd8, Nedd 8, NEDD8, Neddylin, Ubiquitin like protein Nedd8



Tested Applications

Positive WB detected inmouse brain tissue, human heart tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

16777-1-AP targets NEDD8 in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

Tested Reactivity human, mouse, rat, monkey
Cited Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NEDD8 fusion protein Ag10154
Full Name neural precursor cell expressed, developmentally down-regulated 8
Calculated molecular weight 81 aa, 9 kDa
Observed molecular weight 6-10 kDa
GenBank accession numberBC104664
Gene symbol NEDD8
Gene ID (NCBI) 4738
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NEDD8 (neural precursor cell-expressed developmentally down-regulated gene 8) is a small ubiquitin-like protein that shares 60% sequence identity and 80% homology with ubiquitin [PMID:9353319]. NEDD8 is conjugated to substrate proteins in a process known as neddylation. NEDD8 forms a thioester bond with APPBP1-Uba3, the E1 enzyme. Activated NEDD8 then transfers to Ubc12, the E2 enzyme . Ultimately, E2 loaded with NEDD8 binds a substrate protein lysine residue and forms an isopeptide bond by E3 ligase [PMID:18802447].

Protocols

Product Specific Protocols
WB protocol for NEDD8 antibody 16777-1-APDownload protocol
IHC protocol for NEDD8 antibody 16777-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Front Bioeng Biotechnol

Let-7i-5p Regulation of Cell Morphology and Migration Through Distinct Signaling Pathways in Normal and Pathogenic Urethral Fibroblasts.

Authors - Kaile Zhang

J Med Chem

Halo and Pseudohalo Gold(I)-NHC Complexes Derived from 4,5-Diarylimidazoles with Excellent in Vitro and in Vivo Anticancer Activity Against HCC.

Authors - Mianli Bian
rat IF,WB

Neuropsychopharmacology

Synaptic control of DNA methylation involves activity-dependent degradation of DNMT3A1 in the nucleus.

Authors - Gonca Bayraktar
  • KD Validated

Nat Neurosci

Identification of NUB1 as a suppressor of mutant Huntington toxicity via enhanced protein clearance.

Authors - Lu Boxun B

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Jose (Verified Customer) (09-14-2021)

Strong signal

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000