NF-L Polyclonal antibody

NF-L Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 12998-1-AP

Synonyms

68 kDa neurofilament protein, CMT1F, CMT2E, NEFL, NF, NF L, NF68, NFL, NF-L

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Validation Data Gallery

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  IHC staining of mouse brain using 12998-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12998-1-AP (NF-L antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  Ready-to-use IHC kits available with NF-L antibody included
• 

  IHC staining of mouse brain using 12998-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12998-1-AP (NF-L antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  Ready-to-use IHC kits available with NF-L antibody included
• 

  WB analysis of SH-SY5Y using 12998-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 12998-1-AP (NF-L antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain tissue using 12998-1-AP

  WB result of 12998-1-AP.

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  IP experiment of mouse brain using 12998-1-AP

  IP Result of anti-NF-L (IP:12998-1-AP, 4ug; Detection:12998-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

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  IF Staining of SH-SY5Y using 12998-1-AP

  Immunofluorescent analysis of SH-SY5Y cells using 12998-1-AP (NF-L antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of SH-SY5Y using 12998-1-AP

  1X10^6 SH-SY5Y cells were intracellularly stained with 0.2 ug Anti-Human NF-L (12998-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100.

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Tested Applications

• Positive WB detected in: SH-SY5Y cells, mouse brain tissue, Neuro-2a cells
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: SH-SY5Y cells
• Positive FC detected in: SH-SY5Y cells

Planning an IHC experiment? We recommend our IHCeasy NF-L Ready-To-Use IHC Kit. NF-L primary antibody included.

Recommended dilution

• Western Blot (WB): WB : 1:500-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF): IF : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Product Information

12998-1-AP targets NF-L in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NF-L fusion protein Ag3667
• Full Name: neurofilament, light polypeptide
• Calculated molecular weight: 543 aa, 62 kDa
• Observed molecular weight: 65-68 kDa, 140-160 kDa
• GenBank accession number: BC039237
• Gene symbol: NEFL
• Gene ID (NCBI): 4747
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

NEFL, also named as NF68 and NF-L, belongs to the intermediate filament family. Neurofilaments are the 10 nm intermediate filaments found specifically in neurons. They are a major component of the cell's cytoskeleton, and provide support for normal axonal radial growth. Neurofilaments usually contain three intermediate filament proteins: L, M, and H, which are involved in the maintenance of neuronal caliber. The names given to the three major neurofilament subunits are based upon the apparent molecular weight of the mammalian subunits on SDS-PAGE: NF-L, 65-68 kDa; NF-M, 145-160 kDa and NF-H, 200-220 kDa.This antibody is specific to NEFL. It has a very weak reaction to NEFM.

Publications

Species	Application	Title
rat	WB	Neural Regen Res; Bone marrow mesenchymal stem cells repair spinal cord ischemia/reperfusion injury by promoting axonal growth and anti-autophagy.; Authors - Fei Yin
rat	IHC	Neural Regen Res; Inhibiting endogenous tissue plasminogen activator enhanced neuronal apoptosis and axonal injury after traumatic brain injury.; Authors - Jun-Jie Zhao
human		Front Neurol; Serum Protein Biomarker Findings Reflective of Oxidative Stress and Vascular Abnormalities in Male, but Not Female, Collision Sport Athletes.; Authors - Brendan P Major
human	WB,IF	J Tissue Eng Regen Med; Control the fate of human umbilical cord mesenchymal stem cells with dual-enzymatically cross-linked gelatin hydrogels for potential applications in nerve regeneration.; Authors - Jinrui Li
rat	IF	Biochem Pharmacol; Perinatal exposure to nonylphenol delayed myelination in offspring cerebellum.; Authors - Yuanjing Jiang
		Front Mol Neurosci; Achaete-Scute Homolog 1 Expression Controls Cellular Differentiation of Neuroblastoma.; Authors - Mumtaz Kasim

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