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NF-L Antibody 3 Publications

Rabbit Polyclonal| Catalog number: 12998-1-AP

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Con: 65 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, ELISA
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Images See all

NF-L Antibody IHC mouse brain tissue 12998-1-AP

Immunohistochemistry of paraffin-embedded mouse brain tissue slide using 12998-1-AP (NF-L antibody) at dilution of 1:2000 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

NF-L Antibody IHC mouse brain tissue 12998-1-AP

Immunohistochemistry of paraffin-embedded mouse brain tissue slide using 12998-1-AP (NF-L antibody) at dilution of 1:2000 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

NF-L Antibody WB SH-SY5Y cells 12998-1-AP

SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 12998-1-AP (NF-L antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours

NF-L Antibody WB mouse brain tissue 12998-1-AP

WB result of 12998-1-AP.

NF-L Antibody IP mouse brain tissue 12998-1-AP

IP Result of anti-NF-L (IP:12998-1-AP, 4ug; Detection:12998-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

NF-L Antibody IF SH-SY5Y cells 12998-1-AP

Immunofluorescent analysis of SH-SY5Y cells using 12998-1-AP(NF-L antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:
human, mouse, rat
Positive WB detected in:
SH-SY5Y cells, mouse brain tissue, Neuro-2a cells
Positive IP detected in:
mouse brain tissue
Positive IHC detected in:
mouse brain tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
SH-SY5Y cells
Recommended dilution:
WB : 1:500-1:4000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:1000-1:4000
IF : 1:20-1:200

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
NF-L fusion protein Ag3667
Full name:
neurofilament, light polypeptide
Calculated molecular weight:
543aa,62 kDa
Observed molecular weight:
65-68 kDa,140-160 kDa
GenBank accession number:
BC039237
Gene ID (NCBI):
4747
Gene symbol
NEFL
Synonyms
68 kDa neurofilament protein, CMT1F, CMT2E, NEFL, NF L, NF68, NFL, NF-L

Background

NEFL, also named as NF68 and NF-L, belongs to the intermediate filament family. Neurofilaments are the 10 nm intermediate filaments found specifically in neurons. They are a major component of the cell's cytoskeleton, and provide support for normal axonal radial growth. Neurofilaments usually contain three intermediate filament proteins: L, M, and H, which are involved in the maintenance of neuronal caliber. The names given to the three major neurofilament subunits are based upon the apparent molecular weight of the mammalian subunits on SDS-PAGE: NF-L, 65-68 kDa; NF-M, 145-160 kDa and NF-H, 200-220 kDa.This antibody is specific to NEFL. It has a very weak reaction to NEFM.


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