Validation Data Gallery
|Positive WB detected in||SH-SY5Y cells, mouse brain tissue, Neuro-2a cells|
|Positive IP detected in||mouse brain tissue|
|Positive IHC detected in||mouse brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||SH-SY5Y cells|
|Positive FC detected in||SH-SY5Y cells|
|Western Blot (WB)||WB : 1:500-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:1000-1:4000|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
12998-1-AP targets NF-L in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||NF-L fusion protein Ag3667|
|Full Name||neurofilament, light polypeptide|
|Calculated molecular weight||543 aa, 62 kDa|
|Observed molecular weight||65-68 kDa, 140-160 kDa|
|GenBank accession number||BC039237|
|Gene ID (NCBI)||4747|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
NEFL, also named as NF68 and NF-L, belongs to the intermediate filament family. Neurofilaments are the 10 nm intermediate filaments found specifically in neurons. They are a major component of the cell's cytoskeleton, and provide support for normal axonal radial growth. Neurofilaments usually contain three intermediate filament proteins: L, M, and H, which are involved in the maintenance of neuronal caliber. The names given to the three major neurofilament subunits are based upon the apparent molecular weight of the mammalian subunits on SDS-PAGE: NF-L, 65-68 kDa; NF-M, 145-160 kDa and NF-H, 200-220 kDa.This antibody is specific to NEFL. It has a very weak reaction to NEFM.
|Product Specific Protocols|
|WB protocol for NF-L antibody 12998-1-AP||Download protocol|
|IHC protocol for NF-L antibody 12998-1-AP||Download protocol|
|IF protocol for NF-L antibody 12998-1-AP||Download protocol|
|IP protocol for NF-L antibody 12998-1-AP||Download protocol|
|FC protocol for NF-L antibody 12998-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Max (Verified Customer) (02-24-2022)
strong and specific labeling of NfL in hamster brain lysate on western blot