NF-L Polyclonal antibody

NF-L Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

15

Reviews (1)

Cat no : 12998-1-AP

Synonyms

68 kDa neurofilament protein, CMT1F, CMT2E, NEFL, NF, NF L, NF68, NFL, NF-L

---

Validation Data Gallery

• 

  WB analysis of mouse brain tissue using 12998-1-AP

  WB result of 12998-1-AP.

• 

  WB analysis using 12998-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12998-1-AP (NF-L antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 12998-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12998-1-AP (NF-L antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Neuro-2a using 12998-1-AP

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 12998-1-AP (NF-L antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 12998-1-AP

  IP Result of anti-NF-L (IP:12998-1-AP, 4ug; Detection:12998-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

• 

  IHC staining of mouse brain using 12998-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12998-1-AP (NF-L antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 12998-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12998-1-AP (NF-L antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 12998-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12998-1-AP (NF-L antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of SH-SY5Y using 12998-1-AP

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using NF-L antibody (12998-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  FC experiment of SH-SY5Y using 12998-1-AP

  1X10^6 SH-SY5Y cells were intracellularly stained with 0.2 ug Anti-Human NF-L (12998-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100.

---

Tested Applications

• Positive WB detected in: SH-SY5Y cells, Neuro-2a cells, mouse brain tissue, C6 cells
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: SH-SY5Y cells
• Positive FC detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 9 publications below
• IHC: See 1 publications below
• IF: See 5 publications below
• IP: See 1 publications below

Product Information

12998-1-AP targets NF-L in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NF-L fusion protein Ag3667
• Full Name: neurofilament, light polypeptide
• Calculated molecular weight: 543 aa, 62 kDa
• Observed molecular weight: 65-68 kDa, 140-160 kDa
• GenBank accession number: BC039237
• Gene symbol: NEFL
• Gene ID (NCBI): 4747
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

NEFL, also named as NF68 and NF-L, belongs to the intermediate filament family. Neurofilaments are the 10 nm intermediate filaments found specifically in neurons. They are a major component of the cell's cytoskeleton, and provide support for normal axonal radial growth. Neurofilaments usually contain three intermediate filament proteins: L, M, and H, which are involved in the maintenance of neuronal caliber. The names given to the three major neurofilament subunits are based upon the apparent molecular weight of the mammalian subunits on SDS-PAGE: NF-L, 65-68 kDa; NF-M, 145-160 kDa and NF-H, 200-220 kDa.This antibody is specific to NEFL. It has a very weak reaction to NEFM.

Publications

Species	Application	Title
mouse	WB	Mol Cell; Mixed Lineage Kinase Domain-like Protein MLKL Breaks Down Myelin following Nerve Injury.; Authors - Zhengxin Ying
rat	WB,IF	Adv Sci (Weinh); An Important Function of Petrosiol E in Inducing the Differentiation of Neuronal Progenitors and in Protecting Them against Oxidative Stress.; Authors - Jing Liu
		Front Mol Neurosci; Achaete-Scute Homolog 1 Expression Controls Cellular Differentiation of Neuroblastoma.; Authors - Mumtaz Kasim
mouse	WB	Hum Mol Genet; Transverse endoplasmic reticulum expansion in hereditary spastic paraplegia corticospinal axons.; Authors - Peng-Peng Zhu
rat	IF	Biochem Pharmacol; Perinatal exposure to nonylphenol delayed myelination in offspring cerebellum.; Authors - Yuanjing Jiang
mouse	WB,IP	Front Cell Neurosci; A comprehensive mouse brain acetylome-the cellular-specific distribution of acetylated brain proteins; Authors - Yuhua Ji

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Max (Verified Customer) (02-24-2022)

strong and specific labeling of NfL in hamster brain lysate on western blot

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Hamster brain lysate

Related Products

NF-H/NF200 Polyclonal antibody

21471-1-AP

NF-M-Specific Polyclonal antibody

20664-1-AP

NF-M Polyclonal antibody

25805-1-AP

NF-M Monoclonal antibody

66396-1-Ig

NF-H/NF200 Monoclonal antibody

60331-1-Ig

NF-L Monoclonal antibody

60189-1-Ig