NEIL3 Polyclonal antibody, PBS Only

• 

  WB analysis of HEK-293 using 11621-1-AP (same clone as 11621-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11621-1-AP (NEIL3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11621-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 11621-1-AP (same clone as 11621-1-PBS)

  WB result of NEIL3 antibody (11621-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NEIL3 transfected HEK-293 cells. This data was developed using the same antibody clone with 11621-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 11621-1-AP (same clone as 11621-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11621-1-AP (NEIL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11621-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human skin using 11621-1-AP (same clone as 11621-1-PBS)

  human skin tissue were subjected to SDS PAGE followed by western blot with 11621-1-AP (NEIL3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11621-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 11621-1-AP (same clone as 11621-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11621-1-AP (NEIL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11621-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A431 using 11621-1-AP (same clone as 11621-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 11621-1-AP (NEIL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11621-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse skin using 11621-1-AP (same clone as 11621-1-PBS)

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 11621-1-AP (NEIL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11621-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

NEIL3 Polyclonal Antibody for WB, Indirect ELISA

Cat No. 11621-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Indirect ELISA

Synonyms

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Product Information

11621-1-PBS targets NEIL3 in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2200

  Product name: Recombinant human NEIL3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 257-605 aa of BC025954

  Sequence: CHCRITVCRFGDNNRMTYFCPHCQKENPQHVDICKLPTRNTIISWTSSRVDHVMDSVARKSEEHWTCVVCTLINKPSSKACDACLTSRPIDSVLKSEENSTVFSHLMKYPCNTFGKPHTEVKINRKTAFGTTTLVLTDFSNKSSTLERKTKQNQILDEEFQNSPPASVCLNDIQHPSKKTTNDITQLSSKVNISPTISSESKLFSPAHKKPKTAHYSSPELKSCNPGYSNSELQINMTDGPRTLNPDSPRCSKHNRLCILRVVRKDGENKGRQFYACPLPREAQCGFFEWADLSFPFCNHGKRSTMKTVLKIGPNNGKNFFVCPLGKEKQCNFFQWAENGPGIKIIPGC

• Full Name: nei endonuclease VIII-like 3 (E. coli)
• Calculated Molecular Weight: 605 aa, 68 kDa
• Observed Molecular Weight: 62-68 kDa
• GenBank Accession Number: BC025954
• Gene Symbol: NEIL3
• Gene ID (NCBI): 55247
• RRID: AB_2251229
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8TAT5
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

NEIL3 belongs to the FPG family. It is a functional DNA glycosylase in vitro and in vivo. NEIL3 plays a role in repairing FapyG in vivo. (PMID:20185759) NEIL3 has AP lyase activity specific for single-stranded DNA.(PMID:19170771). NEIL3 is specifically expressed in brain areas where neurogenesis takes place during development and that its expression is tightly regulated both temporally and spatially (PMID:19426544). Studies have shown that NEIL3 is recruited to the DSB sites through its GRF zinc finger motifs and plays an important role in MMC/cisplatin-ICL repair by promoting the HR step in FA/BRCA pathway.(PMID: 36351389).