NEIL3 Polyclonal antibody

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NEIL3 Polyclonal Antibody for WB, ELISA

Cat No. 11621-1-AP Copied!

Publications (13)

Reviews (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, ChIP, ELISA

Synonyms

DNA glycosylase FPG2, DNA glycosylase/AP lyase Neil3, EC:3.2.2.-, Endonuclease 8 like 3, Endonuclease 8-like 3

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  WB analysis of HEK-293 using 11621-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11621-1-AP (NEIL3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HEK-293 using 11621-1-AP

  WB result of NEIL3 antibody (11621-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NEIL3 transfected HEK-293 cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 11621-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11621-1-AP (NEIL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human skin using 11621-1-AP

  human skin tissue were subjected to SDS PAGE followed by western blot with 11621-1-AP (NEIL3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HEK-293 using 11621-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11621-1-AP (NEIL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of A431 using 11621-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 11621-1-AP (NEIL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse skin using 11621-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 11621-1-AP (NEIL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HEK-293 cells, A431 cells, mouse skin tissue, human skin tissue, THP-1 cells, U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

11621-1-AP targets NEIL3 in WB, IP, ChIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2200

  Product name: Recombinant human NEIL3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 257-605 aa of BC025954

  Sequence: CHCRITVCRFGDNNRMTYFCPHCQKENPQHVDICKLPTRNTIISWTSSRVDHVMDSVARKSEEHWTCVVCTLINKPSSKACDACLTSRPIDSVLKSEENSTVFSHLMKYPCNTFGKPHTEVKINRKTAFGTTTLVLTDFSNKSSTLERKTKQNQILDEEFQNSPPASVCLNDIQHPSKKTTNDITQLSSKVNISPTISSESKLFSPAHKKPKTAHYSSPELKSCNPGYSNSELQINMTDGPRTLNPDSPRCSKHNRLCILRVVRKDGENKGRQFYACPLPREAQCGFFEWADLSFPFCNHGKRSTMKTVLKIGPNNGKNFFVCPLGKEKQCNFFQWAENGPGIKIIPGC

• Full Name: nei endonuclease VIII-like 3 (E. coli)
• Calculated Molecular Weight: 605 aa, 68 kDa
• Observed Molecular Weight: 62-68 kDa
• GenBank Accession Number: BC025954
• Gene Symbol: NEIL3
• Gene ID (NCBI): 55247
• RRID: AB_2251229
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8TAT5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NEIL3 belongs to the FPG family. It is a functional DNA glycosylase in vitro and in vivo. NEIL3 plays a role in repairing FapyG in vivo. (PMID:20185759) NEIL3 has AP lyase activity specific for single-stranded DNA.(PMID:19170771). NEIL3 is specifically expressed in brain areas where neurogenesis takes place during development and that its expression is tightly regulated both temporally and spatially (PMID:19426544). Studies have shown that NEIL3 is recruited to the DSB sites through its GRF zinc finger motifs and plays an important role in MMC/cisplatin-ICL repair by promoting the HR step in FA/BRCA pathway.(PMID: 36351389).

Publications

What published studies show

The NEIL3 Polyclonal antibody (Cat# 11621-1-AP) has 13 citations. They appear in journals including Nature, Nature Communications, Nucleic Acids Research, EMBO Journal, Cell Reports, Free Radical Biology and Medicine, DNA Repair, Scientific Reports, Frontiers in Cell and Developmental Biology, Discovery Oncology, and Immunogenetics. Research fields span DNA repair (interstrand crosslinks, telomere damage, oxidative damage), cancer biology (ovarian, hepatocellular, prostate cancer), and signaling pathways (Fanconi anemia/BRCA, PI3K/Akt/mTOR).

Automatically generated from the 13 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
	WB	Nature; TRAIP is a master regulator of DNA interstrand crosslink repair.; Authors - R Alex Wu
human	WB	Nat Commun; C/EBPβ enhances platinum resistance of ovarian cancer cells by reprogramming H3K79 methylation.; Authors - Dan Liu
human	WB	Nucleic Acids Res; Cooperation of the NEIL3 and Fanconi anemia/BRCA pathways in interstrand crosslink repair.; Authors - Niu Li ; KD Validated
human	WB	EMBO J; PrimPol-mediated repriming facilitates replication traverse of DNA interstrand crosslinks.; Authors - Daniel González-Acosta
human	WB	Free Radic Biol Med; NEIL1: the second DNA glycosylase involved in action-at-a-distance mutations induced by 8-oxo-7,8-dihydroguanine; Authors - Yoshihiro Fujikawa
human	WB,IP,ChIP	Cell Rep; NEIL3 Repairs Telomere Damage during S Phase to Secure Chromosome Segregation at Mitosis.; Authors - Jia Zhou ; KD Validated

Reviews

4.0 out of 5

1 verified customer reviews

What customers say

The product has received one review with a 4/5 rating. The reviewer found it a good antibody for detecting NEIL3 in human cells, using a dilution of 1:100 for immunoprecipitation and 1:500 for Western blot. The antibody was tested in Western Blot, Immunofluorescence, and Immunoprecipitation applications. Overall, the feedback is positive, indicating reliable performance for NEIL3 detection in human cell systems.

Automatically generated from the 1 verified customer reviews below by Able AI.

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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Jia (Verified Customer) (09-19-2019)

Good antibody for detecting NEIL3 in human cells.

• Applications: Western Blot, Immunofluorescence, Immunoprecipitation,
• Primary Antibody Dilution: 1:100 for IP, 1:500 for WB