NEIL3 Polyclonal antibody

• Featured Product
• KD/KO Validated

NEIL3 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, FC, ChIP, ELISA

Conjugate

Unconjugated

Publications

11

Reviews (1)

Cat no : 11621-1-AP

Synonyms

DNA glycosylase FPG2, DNA glycosylase/AP lyase Neil3, Endonuclease 8 like 3, Endonuclease VIII like 3, FGP2, FPG2, hFPG2, hNEI3, Nei like protein 3, NEI3, NEIL3

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Validation Data Gallery

• 

  WB analysis of HEK-293 using 11621-1-AP

  WB result of NEIL3 antibody (11621-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NEIL3 transfected HEK-293 cells.

• 

  WB analysis of HEK-293 using 11621-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11621-1-AP (NEIL3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11621-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11621-1-AP (NEIL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 11621-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11621-1-AP (NEIL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 11621-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 11621-1-AP (NEIL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skin using 11621-1-AP

  human skin tissue were subjected to SDS PAGE followed by western blot with 11621-1-AP (NEIL3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skin using 11621-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 11621-1-AP (NEIL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HEK-293 cells, A431 cells, mouse skin tissue, human skin tissue, THP-1 cells, U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 11 publications below
• IP: See 3 publications below
• FC: See 1 publications below
• ChIP: See 1 publications below

Product Information

11621-1-AP targets NEIL3 in WB, IP, FC, ChIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NEIL3 fusion protein Ag2200
• Full Name: nei endonuclease VIII-like 3 (E. coli)
• Calculated Molecular Weight: 605 aa, 68 kDa
• Observed Molecular Weight: 62-68 kDa
• GenBank Accession Number: BC025954
• Gene Symbol: NEIL3
• Gene ID (NCBI): 55247
• RRID: AB_2251229
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NEIL3 belongs to the FPG family. It is a functional DNA glycosylase in vitro and in vivo. NEIL3 plays a role in repairing FapyG in vivo. (PMID:20185759) NEIL3 has AP lyase activity specific for single-stranded DNA.(PMID:19170771). NEIL3 is specifically expressed in brain areas where neurogenesis takes place during development and that its expression is tightly regulated both temporally and spatially (PMID:19426544). Studies have shown that NEIL3 is recruited to the DSB sites through its GRF zinc finger motifs and plays an important role in MMC/cisplatin-ICL repair by promoting the HR step in FA/BRCA pathway.(PMID: 36351389).

Publications

Species	Application	Title
human	WB	Nat Commun; C/EBPβ enhances platinum resistance of ovarian cancer cells by reprogramming H3K79 methylation.; Authors - Dan Liu
human	WB	Nucleic Acids Res; Cooperation of the NEIL3 and Fanconi anemia/BRCA pathways in interstrand crosslink repair.; Authors - Niu Li ; KD Validated
human	WB	EMBO J; PrimPol-mediated repriming facilitates replication traverse of DNA interstrand crosslinks.; Authors - Daniel González-Acosta
human	WB	Sci Rep; NEIL3-mediated proteasomal degradation facilitates the repair of cisplatin-induced DNA damage in human cells; Authors - Umit Aliyaskarova
human	WB,IP	DNA Repair (Amst); Release from quiescence stimulates the expression of human NEIL3 under the control of the Ras dependent ERK-MAP kinase pathway.; Authors - Neurauter Christine Gran CG
human	WB,FC,IP,ChIP	Cell Rep; NEIL3 Repairs Telomere Damage during S Phase to Secure Chromosome Segregation at Mitosis.; Authors - Jia Zhou ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Jia (Verified Customer) (09-19-2019)

Good antibody for detecting NEIL3 in human cells.

• Applications: Western Blot, Immunofluorescence, Immunoprecipitation,
• Primary Antibody Dilution: 1:100 for IP, 1:500 for WB