NEK7 Polyclonal antibody

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  WB analysis using 31422-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31422-1-AP (NEK7 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 31422-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31422-1-AP (NEK7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

NEK7 Polyclonal Antibody for WB, ELISA

Cat No. 31422-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

NimA related protein kinase 7

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Tested Applications

• Positive WB detected in: C6 cells, HeLa cells, Jurkat cells, NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31422-1-AP targets NEK7 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NEK7 fusion protein Ag33629
• Full Name: NIMA (never in mitosis gene a)-related kinase 7
• Calculated Molecular Weight: 35
• Observed Molecular Weight: 32-35 kDa
• GenBank Accession Number: NM_133494
• Gene Symbol: NEK7
• Gene ID (NCBI): 140609
• RRID: AB_3669975
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q8TDX7
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Mammalian NIMA-related kinases (NEKs) represent a family of serine/threonine kinases, named NEK1-NEK11, which are implicated in the control of several aspects of mitosis and are involved in non-mitotic functions (PMID: 33364979). NIMA-related kinase 7 (NEK7) is the smallest NIMA-related kinase (NEK) in mammals. NEK7 is identified as a highly conserved serine or threonine kinase, which is not only crucial for mitosis entry, cell cycle progression, cell division, and mitotic process, but also expressed in brain, heart, lung, liver, spleen, and other issues (PMID: 31787755).