NHE1 Monoclonal antibody

NHE1 Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

CloneNo.

3D9F3

Cat no : 67363-1-Ig

Synonyms

APNH, APNH1, FLJ42224, Na(+)/H(+) exchanger 1, NHE 1, NHE1, SLC9A1, Sodium/hydrogen exchanger 1



Tested Applications

Positive WB detected inA549 cells, NCI-H1299 cells, HEK-293 cells, LNCaP cells, Jurkat cells, HSC-T6 cells, PC-12 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

67363-1-Ig targets NHE1 in WB, IHC,ELISA applications and shows reactivity with Human, Rat samples.

Tested Reactivity Human, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NHE1 fusion protein Ag14329
Full Name solute carrier family 9 (sodium/hydrogen exchanger), member 1
Calculated molecular weight 815 aa, 91 kDa
Observed molecular weight 110 kDa
GenBank accession numberBC012121
Gene symbol SLC9A1
Gene ID (NCBI) 6548
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

NHE1, also named as SLC9A1, is involved in pH regulation that can eliminate acids generated by active metabolism and counter adverse environmental conditions. NHE1 plays an important role in signal transduction. It has been shown that NHE1 participates in the development and progression of human breast cancer, gastric cancer and others. NHE1 has some isoforms with 91 kDa , 110 kDa (phosphoglycoprotein) and 210 kDa (dimeric protein). (PMID:28098891, 27751915, 8300588)

Protocols

Product Specific Protocols
WB protocol for NHE1 antibody 67363-1-IgDownload protocol
IHC protocol for NHE1 antibody 67363-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols