NIPBL Polyclonal antibody, PBS Only

• 

  WB analysis of HeLa using 18792-1-AP (same clone as 18792-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 18792-1-AP (NIPBL antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18792-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 18792-1-AP (same clone as 18792-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 18792-1-AP (NIPBL antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18792-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 18792-1-AP (same clone as 18792-1-PBS)

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 18792-1-AP (NIPBL antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 18792-1-PBS in a different storage buffer formulation.

NIPBL Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 18792-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

---

Product Information

18792-1-PBS targets NIPBL in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5663

  Product name: Recombinant human NIPBL protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-175 aa of BC033847

  Sequence: MKCLPENSAPLIEFANVSQGILLLLMLKQHLKNLCGFSDSKIQKYSPSESAKVYDKAINRKTGVHFHPKQTLDFLRSDMANSKITEEVKRSIVKQYLDFKLLMEHLDPDEEEEEGEVSASTNARNKAITSLLGGGSPKNNTAAETEDDESDGEDRGGGTSGVRRRRSQRISQRIT

• Full Name: Nipped-B homolog (Drosophila)
• Calculated Molecular Weight: 2804 aa, 316 kDa
• Observed Molecular Weight: 300 kDa
• GenBank Accession Number: BC033847
• Gene Symbol: NIPBL
• Gene ID (NCBI): 25836
• RRID: AB_2151288
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q6KC79
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

NIPBL, also named as Nipped-B-like protein, is a 28-4 amino acid protein, which Contains one Pro-Xaa-Val-Xaa-Leu (PxVxL) motif, which is required for interaction with chromoshadow domains and belongs to the SCC2/Nipped-B family. NIPBL probably plays a structural role in chromatin and Involve in sister chromatid cohesion, possibly by interacting with the cohesin complex. endogenous full length NIPBL which is estimated to be about 300kDa led us to believe that the NIPBL protein was deteriorated or lost by the standard protein extraction and blotting techniques.