NIPBL Polyclonal antibody

NIPBL Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ChIP, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 18792-1-AP

Synonyms

CDLS, CDLS1, Delangin, IDN3, IDN3 B, NIPBL, Nipped B homolog (Drosophila), Nipped B like protein, SCC2 homolog

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Validation Data Gallery

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  WB analysis of HeLa using 18792-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18792-1-AP (NIPBL antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 18792-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18792-1-AP (NIPBL antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 18792-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 18792-1-AP (NIPBL antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IP: See 1 publications below
• ChIP: See 2 publications below

Product Information

18792-1-AP targets NIPBL in WB, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NIPBL fusion protein Ag5663
• Full Name: Nipped-B homolog (Drosophila)
• Calculated Molecular Weight: 2804 aa, 316 kDa
• Observed Molecular Weight: 300 kDa
• GenBank Accession Number: BC033847
• Gene Symbol: NIPBL
• Gene ID (NCBI): 25836
• RRID: AB_2151288
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NIPBL, also named as Nipped-B-like protein, is a 28-4 amino acid protein, which Contains one Pro-Xaa-Val-Xaa-Leu (PxVxL) motif, which is required for interaction with chromoshadow domains and belongs to the SCC2/Nipped-B family. NIPBL probably plays a structural role in chromatin and Involve in sister chromatid cohesion, possibly by interacting with the cohesin complex. endogenous full length NIPBL which is estimated to be about 300kDa led us to believe that the NIPBL protein was deteriorated or lost by the standard protein extraction and blotting techniques.

Publications

Species	Application	Title
mouse	ChIP	Cell; Lineage-specific 3D genome organization is assembled at multiple scales by IKAROS; Authors - Yeguang Hu
human	WB	Nat Commun; C/EBPβ enhances platinum resistance of ovarian cancer cells by reprogramming H3K79 methylation.; Authors - Dan Liu
mouse	ChIP	Nat Immunol; BRWD1 orchestrates small pre-B cell chromatin topology by converting static to dynamic cohesin; Authors - Malay Mandal
mouse	WB,IP	Nucleic Acids Res; BRD2 promotes antibody class switch recombination by facilitating DNA repair in collaboration with NIPBL; Authors - Santosh K Gothwal