CoraLite® Plus 647 Anti-Mouse NKp46/NCR1 Rabbit Recombinant Antibody

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  FC experiment of mouse splenocytes using CL647-98198

  1x10^6 mouse splenocytes were surface stained with CoraLite® Plus 488 Anti-Mouse CD49b (DX5) (CL488-65065, Clone: DX5), and 0.25 ug CoraLite® Plus 647 Anti-Mouse NKp46/NCR1 Rabbit RecAb (CL647-98198, Clone:241804A12) or CoraLite® Plus 647 Rabbit IgG Isotype Control RecAb (CL647-98136, Clone: 240953C9). Cells were not fixed.

• 

  FC experiment of mouse splenocytes using CL647-98198

  1x10^6 mouse splenocytes were surface stained with 0.25 ug CoraLite® Plus 647 Anti-Mouse NKp46/NCR1 Rabbit RecAb (CL647-98198, Clone: 241804A12) (red) or CoraLite® Plus 647 Rabbit IgG Isotype Control RecAb (CL647-98136, Clone: 240953C9) (blue). Cells were not fixed.

NKp46/NCR1 Uni-rAb^TM Recombinant Antibody for FC

Cat No. CL647-98198

Clone No.241804A12

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Synonyms

Ncr1, 241804A12, Activating receptor 1, CD335, Ly94

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Tested Applications

• Positive FC detected in: mouse splenocytes

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-98198 targets NKp46/NCR1 in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: NKp46/NCR1 fusion protein Eg1825
• Full Name: natural cytotoxicity triggering receptor 1
• Calculated Molecular Weight: 37kDa
• GenBank Accession Number: NM_010746.3
• Gene Symbol: NKp46/NCR1
• Gene ID (NCBI): 17086
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q8C567
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

NKp46, also known as NCR1 or CD335, is an Ig-like superfamily cell surface receptor that is highly conserved in mammals (PMID: 22021440). NKp46 is a type I transmembrane glycoprotein consisting of two extracellular Ig-like domains, a transmembrane domain, and an intracellular tail (PMID: 9730896). It is expressed on NK cells, rare T-cell subsets and a mucosal population of NKp46+ innate lymphoid cells (PMID: 9730896; 22021440). NKp46 is the major triggering receptor involved in the natural cytotoxicity (PMID: 10359120; 10092106; 15356098).