FITC Plus Anti-Mouse NKp46/NCR1 Rabbit Recombinant Antibody

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  FC experiment of mouse splenocytes using FITC-98198

  1x10^6 mouse splenocytes were surface stained with APC Anti-Mouse CD49b (DX5) (APC-65065, Clone: DX5), and 0.25 ug FITC Plus Anti-Mouse NKp46/NCR1 Rabbit RecAb (FITC-98198, Clone: 241804A12) or FITC Plus Rabbit IgG Isotype Control RecAb (FITC-98136, Clone: 240953C9). Cells were not fixed.

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  FC experiment of mouse splenocytes using FITC-98198

  1x10^6 mouse splenocytes were surface stained with 0.25 ug FITC Plus Anti-Mouse NKp46/NCR1 Rabbit RecAb (FITC-98198, Clone: 241804A12) (red) or FITC Plus Rabbit IgG Isotype Control RecAb (FITC-98136, Clone: 240953C9) (blue). Cells were not fixed.

NKp46/NCR1 Uni-rAb^TM Recombinant Antibody for FC

Cat No. FITC-98198

Clone No.241804A12

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Synonyms

Ncr1, 241804A12, Activating receptor 1, CD335, Ly94

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Tested Applications

• Positive FC detected in: mouse splenocytes

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

FITC-98198 targets NKp46/NCR1 in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: NKp46/NCR1 fusion protein Eg1825
• Full Name: natural cytotoxicity triggering receptor 1
• Calculated Molecular Weight: 37kDa
• GenBank Accession Number: NM_010746.3
• Gene Symbol: NKp46/NCR1
• Gene ID (NCBI): 17086
• Conjugate: FITC Plus Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 495 nm / 524 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q8C567
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

NKp46, also known as NCR1 or CD335, is an Ig-like superfamily cell surface receptor that is highly conserved in mammals (PMID: 22021440). NKp46 is a type I transmembrane glycoprotein consisting of two extracellular Ig-like domains, a transmembrane domain, and an intracellular tail (PMID: 9730896). It is expressed on NK cells, rare T-cell subsets and a mucosal population of NKp46+ innate lymphoid cells (PMID: 9730896; 22021440). NKp46 is the major triggering receptor involved in the natural cytotoxicity (PMID: 10359120; 10092106; 15356098).