NMDAR1 Polyclonal antibody

NMDAR1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27232-1-AP

Synonyms

GluN1, GRIN1, NMDAR1, NR1

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Validation Data Gallery

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  WB analysis using 27232-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27232-1-AP (NMDAR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of rat brain using 27232-1-AP

  IP result of anti-NMDAR1 (IP:27232-1-AP, 4ug; Detection:27232-1-AP 1:400) with rat brain tissue lysate 1120 ug.

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  IHC staining of mouse cerebellum using 27232-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 27232-1-AP (NMDAR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 27232-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 27232-1-AP (NMDAR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 27232-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27232-1-AP (NMDAR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 27232-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27232-1-AP (NMDAR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of rat brain using 27232-1-AP

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using NMDAR1 antibody (27232-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of rat brain using 27232-1-AP

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using NMDAR1 antibody (27232-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IP detected in: rat brain tissue
• Positive IHC detected in: mouse brain tissue, mouse cerebellum tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27232-1-AP targets NMDAR1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NMDAR1 fusion protein Ag26093
• Full Name: glutamate receptor, ionotropic, N-methyl D-aspartate 1
• Calculated Molecular Weight: 105 kDa
• Observed Molecular Weight: 116-120 kDa
• GenBank Accession Number: NM_000832
• Gene Symbol: GRIN1
• Gene ID (NCBI): 2902
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GRIN1 encodes subunit 1 of the N-methyl-D-aspartate (NMDA) receptor, which is a heteromeric glutamate-gated calcium ion channel essential for synaptic function in the brain (PMID: 25864721, PMID: 25864721). NMDARs play important roles in normal brain development and function, such as synaptic plasticity, neural development, learning and memory (PMID: 20716669). NMDAR dysfunction has been associated with several neurological disorders including Parkinson, Alzheimer and Huntington diseases. Disrupted motor learning and long-term synaptic plasticity in mice lacking NMDAR1 in the striatum (PMID: 17015831).