NMDAR2A/GRIN2A Polyclonal antibody, PBS Only

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  WB analysis using 28571-1-AP (same clone as 28571-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 28571-1-AP (NMDAR2A/GRIN2A antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28571-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 28571-1-AP (same clone as 28571-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28571-1-AP (NMDAR2A/GRIN2A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28571-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 28571-1-AP (same clone as 28571-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28571-1-AP (NMDAR2A/GRIN2A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28571-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 28571-1-AP (same clone as 28571-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 28571-1-AP (NMDAR2A/GRIN2A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28571-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 28571-1-AP (same clone as 28571-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 28571-1-AP (NMDAR2A/GRIN2A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28571-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 28571-1-AP (same clone as 28571-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using NMDAR2A/GRIN2A antibody (28571-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 28571-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse brain using 28571-1-AP (same clone as 28571-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using NMDAR2A/GRIN2A antibody (28571-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 28571-1-PBS in a different storage buffer formulation.

• 

  IF Staining of rat brain using 28571-1-AP (same clone as 28571-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded rat brain tissue using NMDAR2A/GRIN2A antibody (28571-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 28571-1-PBS in a different storage buffer formulation.

NMDAR2A/GRIN2A Polyclonal Antibody for WB, IHC, IF-P, IF-Fro, Indirect ELISA

Cat No. 28571-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, IF-P, IF-Fro, Indirect ELISA

Synonyms

GRIN2A, NMDAR2A, glun2a, Glutamate [NMDA] receptor subunit epsilon-1, Glutamate receptor ionotropic, NMDA 2A

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Product Information

28571-1-PBS targets NMDAR2A/GRIN2A in WB, IHC, IF-P, IF-Fro, Indirect ELISA applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag29423

  Product name: Recombinant human NMDAR2A/GRIN2A protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1000-1127 aa of NM_000833

  Sequence: VSTESKANSRPRQLWKKSVDSIRQDSLSQNPVSQRDEATAENRTHSLKSPRYLPEEMAHSDISETSNRATCHREPDNSKNHKTKDNFKRSVASKYPKDCSEVERTYLKTKSSSPRDKIYTIDGEKEPG

• Full Name: glutamate receptor, ionotropic, N-methyl D-aspartate 2A
• Calculated Molecular Weight: 165 kDa
• Observed Molecular Weight: 180 kDa
• GenBank Accession Number: NM_000833
• Gene Symbol: GRIN2A
• Gene ID (NCBI): 2903
• RRID: AB_2881171
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q12879
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.