NMDAR2A/GRIN2A Polyclonal antibody

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  WB analysis using 28571-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28571-1-AP (NMDAR2A/GRIN2A antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 28571-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28571-1-AP (NMDAR2A/GRIN2A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 28571-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28571-1-AP (NMDAR2A/GRIN2A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 28571-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 28571-1-AP (NMDAR2A/GRIN2A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 28571-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 28571-1-AP (NMDAR2A/GRIN2A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 28571-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using NMDAR2A/GRIN2A antibody (28571-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse brain using 28571-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using NMDAR2A/GRIN2A antibody (28571-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of rat brain using 28571-1-AP

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded rat brain tissue using NMDAR2A/GRIN2A antibody (28571-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

NMDAR2A/GRIN2A Polyclonal Antibody for WB, IHC, IF-P, IF-Fro, ELISA

Cat No. 28571-1-AP

Publications(5)

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, IF-P, IF-Fro, ELISA

Synonyms

GRIN2A, NMDAR2A, glun2a, Glutamate [NMDA] receptor subunit epsilon-1, Glutamate receptor ionotropic, NMDA 2A

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IHC detected in: mouse brain tissue, mouse cerebellum tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse brain tissue
• Positive IF-Fro detected in: rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)-FRO: IF-FRO : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IF: See 1 publications below

Product Information

28571-1-AP targets NMDAR2A/GRIN2A in WB, IHC, IF-P, IF-Fro, ELISA applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Cited Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag29423

  Product name: Recombinant human NMDAR2A/GRIN2A protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1000-1127 aa of NM_000833

  Sequence: VSTESKANSRPRQLWKKSVDSIRQDSLSQNPVSQRDEATAENRTHSLKSPRYLPEEMAHSDISETSNRATCHREPDNSKNHKTKDNFKRSVASKYPKDCSEVERTYLKTKSSSPRDKIYTIDGEKEPG

• Full Name: glutamate receptor, ionotropic, N-methyl D-aspartate 2A
• Calculated Molecular Weight: 165 kDa
• Observed Molecular Weight: 180 kDa
• GenBank Accession Number: NM_000833
• Gene Symbol: GRIN2A
• Gene ID (NCBI): 2903
• RRID: AB_2881171
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q12879
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Behav Brain Res; Down-regulation of NR2B receptors contributes to the analgesic and antianxiety effects of enriched environment mediated by endocannabinoid system in the inflammatory pain mice; Authors - Shukun Jiang
rat	WB	Heliyon; Cherry leaf decoction inhibits NMDAR expression and thereby ameliorates CUMS- induced depression-like behaviors through downregulation of α2δ-1; Authors - Chuan Jiang
mouse	WB	Neurochem Res; Scavenger Receptor Class B Type I Modulates Epileptic Seizures and Receptor α2δ-1 Expression; Authors - Yunyi Huang
mouse	WB	J Ethnopharmacol; Anticonvulsant effect of Stachydrine on pentylenetetrazole-induced kindling seizure mouse model via Notch and NMDAR signaling pathways; Authors - Qifa Li
mouse	IF	Neuron; The paraventricular thalamus mediates visceral pain and anxiety-like behaviors via two distinct pathways; Authors - Di Li