NMDAR2A/GRIN2A Polyclonal antibody

NMDAR2A/GRIN2A Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 28571-1-AP

Synonyms

GRIN2A, hNR2A, NMDAR2A/GRIN2A, NR2A



Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue, mouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 2 publications below

Product Information

28571-1-AP targets NMDAR2A/GRIN2A in WB, IHC, IF, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Cited Reactivitymouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NMDAR2A/GRIN2A fusion protein Ag29423
Full Name glutamate receptor, ionotropic, N-methyl D-aspartate 2A
Calculated molecular weight 165 kDa
Observed molecular weight180 kDa
GenBank accession numberNM_000833
Gene symbol GRIN2A
Gene ID (NCBI) 2903
RRIDAB_2881171
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for NMDAR2A/GRIN2A antibody 28571-1-APDownload protocol
IHC protocol for NMDAR2A/GRIN2A antibody 28571-1-APDownload protocol
IF protocol for NMDAR2A/GRIN2A antibody 28571-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Behav Brain Res

Down-regulation of NR2B receptors contributes to the analgesic and antianxiety effects of enriched environment mediated by endocannabinoid system in the inflammatory pain mice

Authors - Shukun Jiang
ratWB

Heliyon

Cherry leaf decoction inhibits NMDAR expression and thereby ameliorates CUMS- induced depression-like behaviors through downregulation of α2δ-1

Authors - Chuan Jiang