NOB1 Polyclonal antibody

• Featured Product
• KD/KO Validated

NOB1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 10091-2-AP

Synonyms

NOB1P, MSTP158, MST158, EC:3.1.-.-, ART4

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Validation Data Gallery

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  WB analysis using 10091-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10091-2-AP (NOB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of SGC-7901 using 10091-2-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 10091-2-AP (NOB1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 10091-2-AP

  IP result of anti-NOB1 (IP:10091-2-AP, 4ug; Detection:10091-2-AP 1:300) with HepG2 cells lysate 2400ug.

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  IHC staining of human lymphoma using 10091-2-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 10091-2-AP (NOB1 antibody) at dilution of 1:25 (under 10x lens).

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  IHC staining of human placenta using 10091-2-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10091-2-AP (NOB1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 10091-2-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10091-2-AP (NOB1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 10091-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10091-2-AP (NOB1 antibody) at dilution of 1:25 (under 10x lens).

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  IHC staining of human colon cancer using 10091-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10091-2-AP (NOB1 antibody) at dilution of 1:25 (under 40x lens).

• 

  IF Staining of A549 using 10091-2-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using NOB1 antibody (10091-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, SGC-7901 cells, HepG2 cells, K-562 cells
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human lymphoma tissue, human placenta tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:10-1:100
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IHC: See 1 publications below

Product Information

10091-2-AP targets NOB1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NOB1 fusion protein Ag0141
• Full Name: NIN1/RPN12 binding protein 1 homolog (S. cerevisiae)
• Calculated Molecular Weight: 47 kDa
• Observed Molecular Weight: 47-50 kDa
• GenBank Accession Number: BC000050
• Gene Symbol: NOB1
• Gene ID (NCBI): 28987
• RRID: AB_2298640
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NOB1 was first identified in yeast as an essential gene encoding the Nin one binding protein, which is involved in pre-rRNA processing. In a late cytoplasmic processing step, Nob1 cleaves a 20S rRNA intermediate at cleavage site D to produce the mature 18S rRNA. In addition, NOB1 is a crucial molecule in the maturation of the 20S proteasome and protein degradation. It serves as a chaperone to join the 20S proteasome with the 19S regulatory particle in the nucleus and facilitates the maturation of the 20S proteasome. Recently NOB1 has been reported to be overexpressed in several types of cancer, suggesting its involvement in the tumorigenesis.

Publications

Species	Application	Title
	WB	Nat Commun; Molecular basis for disassembly of an importin:ribosomal protein complex by the escortin Tsr2.; Authors - Sabina Schütz
	WB	Elife; A RanGTP-independent mechanism allows ribosomal protein nuclear import for ribosome assembly.; Authors - Sabina Schütz
	WB	Elife; Prefabrication of a ribosomal protein subcomplex essential for eukaryotic ribosome formation.; Authors - Cohue Peña
human	WB	World J Gastroenterol; NOB1 is essential for the survival of RKO colorectal cancer cells.; Authors - Xiao-Wen He ; KD Validated
		Oncol Rep; NOB1 silencing inhibits the growth and metastasis of laryngeal cancer cells through the regulation of JNK signaling pathway.; Authors - Xin Gao
human	WB	Oncol Lett; Effects of NOB1 on the pathogenesis of osteosarcoma and its expression on the chemosensitivity to cisplatin.; Authors - Leiming Luo ; KD Validated