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  • KD/KO Validated

NOB1 Polyclonal antibody

NOB1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10091-2-AP

Synonyms

ART 4, ART4, MST158, MSTP158, NOB1, NOB1P, Protein ART 4, PSMD8BP1, RNA binding protein NOB1



Tested Applications

Positive WB detected inHeLa cells, SGC-7901 cells, HepG2 cells, K-562 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman lymphoma tissue, human placenta tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:10-1:100
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

10091-2-AP targets NOB1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NOB1 fusion protein Ag0141
Full Name NIN1/RPN12 binding protein 1 homolog (S. cerevisiae)
Calculated molecular weight 47 kDa
Observed molecular weight 47-50 kDa
GenBank accession numberBC000050
Gene symbol NOB1
Gene ID (NCBI) 28987
RRIDAB_2298640
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NOB1 was first identified in yeast as an essential gene encoding the Nin one binding protein, which is involved in pre-rRNA processing. In a late cytoplasmic processing step, Nob1 cleaves a 20S rRNA intermediate at cleavage site D to produce the mature 18S rRNA. In addition, NOB1 is a crucial molecule in the maturation of the 20S proteasome and protein degradation. It serves as a chaperone to join the 20S proteasome with the 19S regulatory particle in the nucleus and facilitates the maturation of the 20S proteasome. Recently NOB1 has been reported to be overexpressed in several types of cancer, suggesting its involvement in the tumorigenesis.

Protocols

Product Specific Protocols
WB protocol for NOB1 antibody 10091-2-APDownload protocol
IHC protocol for NOB1 antibody 10091-2-APDownload protocol
IF protocol for NOB1 antibody 10091-2-APDownload protocol
IP protocol for NOB1 antibody 10091-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Nat Commun

Molecular basis for disassembly of an importin:ribosomal protein complex by the escortin Tsr2.

Authors - Sabina Schütz
WB

Elife

A RanGTP-independent mechanism allows ribosomal protein nuclear import for ribosome assembly.

Authors - Sabina Schütz
WB

Elife

Prefabrication of a ribosomal protein subcomplex essential for eukaryotic ribosome formation.

Authors - Cohue Peña
humanWB

World J Gastroenterol

NOB1 is essential for the survival of RKO colorectal cancer cells.

Authors - Xiao-Wen He
  • KD Validated

Oncol Rep

NOB1 silencing inhibits the growth and metastasis of laryngeal cancer cells through the regulation of JNK signaling pathway.

Authors - Xin Gao
humanWB

Oncol Lett

Effects of NOB1 on the pathogenesis of osteosarcoma and its expression on the chemosensitivity to cisplatin.

Authors - Leiming Luo
  • KD Validated