Validation Data Gallery
|Positive WB detected in
|HeLa cells, SGC-7901 cells, HepG2 cells, K-562 cells
|Positive IP detected in
|Positive IHC detected in
|human lymphoma tissue, human placenta tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|Western Blot (WB)
|WB : 1:500-1:2000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:10-1:100
|IF : 1:200-1:800
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
10091-2-AP targets NOB1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.
|Host / Isotype
|Rabbit / IgG
|NOB1 fusion protein Ag0141
|NIN1/RPN12 binding protein 1 homolog (S. cerevisiae)
|Calculated molecular weight
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
NOB1 was first identified in yeast as an essential gene encoding the Nin one binding protein, which is involved in pre-rRNA processing. In a late cytoplasmic processing step, Nob1 cleaves a 20S rRNA intermediate at cleavage site D to produce the mature 18S rRNA. In addition, NOB1 is a crucial molecule in the maturation of the 20S proteasome and protein degradation. It serves as a chaperone to join the 20S proteasome with the 19S regulatory particle in the nucleus and facilitates the maturation of the 20S proteasome. Recently NOB1 has been reported to be overexpressed in several types of cancer, suggesting its involvement in the tumorigenesis.
|Product Specific Protocols
|WB protocol for NOB1 antibody 10091-2-AP
|IHC protocol for NOB1 antibody 10091-2-AP
|IF protocol for NOB1 antibody 10091-2-AP
|IP protocol for NOB1 antibody 10091-2-AP
|Click here to view our Standard Protocols
Molecular basis for disassembly of an importin:ribosomal protein complex by the escortin Tsr2.
A RanGTP-independent mechanism allows ribosomal protein nuclear import for ribosome assembly.
Prefabrication of a ribosomal protein subcomplex essential for eukaryotic ribosome formation.
World J Gastroenterol
NOB1 is essential for the survival of RKO colorectal cancer cells.
NOB1 silencing inhibits the growth and metastasis of laryngeal cancer cells through the regulation of JNK signaling pathway.
Effects of NOB1 on the pathogenesis of osteosarcoma and its expression on the chemosensitivity to cisplatin.