iNOS Polyclonal antibody

• Featured Product
• KD/KO Validated

iNOS Polyclonal Antibody for FC, IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IP, IF, FC, ELISA

Conjugate

Unconjugated

Publications

456

Reviews (4)

Cat no : 18985-1-AP

Synonyms

HEP NOS, Hepatocyte NOS, Inducible NO synthase, Inducible NOS, INOS, NOS, NOS type II, NOS2, NOS2A

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Validation Data Gallery

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  WB analysis using 18985-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18985-1-AP (iNOS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 18985-1-AP

  WB result of iNOS antibody (18985-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-iNOS transfected HeLa cells.

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  WB analysis of HeLa using 18985-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18985-1-AP (iNOS antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 18985-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using iNOS antibody (18985-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 18985-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 18985-1-AP (iNOS antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HepG2 using 18985-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human iNOS (18985-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: mouse liver tissue, rat liver tissue
• Positive IF detected in: HepG2 cells, HeLa cells
• Positive FC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:4000
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 338 publications below
• IHC: See 61 publications below
• IF: See 126 publications below
• IP: See 1 publications below
• ELISA: See 2 publications below
• FC: See 1 publications below

Product Information

18985-1-AP targets iNOS in WB, IP, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, chicken, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: nitric oxide synthase 2, inducible
• Calculated molecular weight: 131 kDa
• Observed molecular weight: 110-130 kDa, 65-70 kDa
• GenBank accession number: NM_000625
• Gene symbol: NOS2
• Gene ID (NCBI): 4843
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NOS2, also named as iNOS and NOS2A, produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. NO is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission, antimicrobial and antitumoral activities. NOS2 is a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. iNOS has a very short half-life due to rapid degradation by calpain. iNOS monomer is a direct substrate of calpain I and can be cleaved by calpain I at the canonical CaM-binding site(503-532aa) of iNOS, and then a ~70 kDa band can be detected by western (PMID:11786228). This antibody is specific to NOS2.

Publications

Species	Application	Title
human	WB	J Extracell Vesicles; Extracellular vesicle-mediated delivery of circDYM alleviates CUS-induced depressive-like behaviours.; Authors - Xiaoyu Yu
mouse	WB	Redox Biol; Icariin alleviates uveitis by targeting peroxiredoxin 3 to modulate retinal microglia M1/M2 phenotypic polarization.; Authors - Guoqing Wang
mouse	IF	JHEP Rep; TAK1 deficiency promotes liver injury and tumorigenesis via ferroptosis and macrophage cGAS-STING signalling; Authors - Wantong Su
mouse	WB	Autophagy; PARP14 inhibits microglial activation via LPAR5 to promote post-stroke functional recovery.; Authors - Ying Tang
human	IHC	Bioact Mater; Facile fabrication of a biocompatible composite gel with sustained release of aspirin for bone regeneration.; Authors - Yunfan Zhang
rat	IF	Bioact Mater; High proportion strontium-doped micro-arc oxidation coatings enhance early osseointegration of titanium in osteoporosis by anti-oxidative stress pathway.; Authors - Xinkun Shen

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Yasuyo (Verified Customer) (03-01-2022)

This is one of the best IHC signaling I ever got for iNOS. Amazing image. I recommend this for iNOS detection by IHC.

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:250
• Cell Tissue Type: skin

iNOS Antibody Immunohistochemistry validation (1:250 dilution) in skin (Cat no:18985-1-AP)

FH

Xie (Verified Customer) (10-06-2021)

Works nice for WB assay.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: RAW 264.7

iNOS Antibody Western Blot validation (1:500 dilution) in RAW 264.7 (Cat no:18985-1-AP)

FH

Prasanth (Verified Customer) (08-19-2019)

This is one of the cleanest and clarity blot I ever got for iNOS. Amazing clarity and no non specific bands. I recommend this for iNOS detection by western blot.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse Fibroblast Cells

FH

Elena (Verified Customer) (08-14-2019)

We tried this antibody with 2 cell lines and it worked just fine. We will use this in the future for other cell lines as well. The antibody produced strong bands at the suggested concentration and we diluted it further.

• Applications: Western Blot,

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