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  • KD/KO Validated

iNOS Polyclonal antibody

iNOS Polyclonal Antibody for FC, IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IP, IF, FC

Conjugate

Unconjugated

Cat no : 18985-1-AP

Synonyms

HEP NOS, Hepatocyte NOS, Inducible NO synthase, Inducible NOS, INOS, NOS, NOS type II, NOS2, NOS2A



Tested Applications

Positive WB detected inmouse liver tissue, rat liver tissue
Positive IF detected inHepG2 cells, HeLa cells
Positive FC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:4000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

18985-1-AP targets iNOS in WB, IP, IF, FC applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity chicken, human, mouse, pig, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name nitric oxide synthase 2, inducible
Calculated molecular weight 131 kDa
Observed molecular weight 110-130 kDa, 65-70 kDa
GenBank accession numberNM_000625
Gene symbol NOS2
Gene ID (NCBI) 4843
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NOS2, also named as iNOS and NOS2A, produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. NO is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission, antimicrobial and antitumoral activities. NOS2 is a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. iNOS has a very short half-life due to rapid degradation by calpain. iNOS monomer is a direct substrate of calpain I and can be cleaved by calpain I at the canonical CaM-binding site(503-532aa) of iNOS, and then a ~70kD band can be detected by western (PMID:11786228). This antibody is specific to NOS2.

Protocols

Product Specific Protocols
WB protocol for iNOS antibody 18985-1-APDownload protocol
IF protocol for iNOS antibody 18985-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Biol Trace Elem Res

Mechanism Underlying the Protective Effect of Selenium on NO-Induced Oxidative Damage in Bovine Mammary Epithelial Cells.

Authors - Yongmei Guo
ratWB,IHC

Iran J Basic Med Sci

Tempol relieves lung injury in a rat model of chronic intermittent hypoxia via suppression of inflammation and oxidative stress.

Authors - Yeying Wang
mouseWB

Oxid Med Cell Longev

Flavonoid-Rich Ethanol Extract from the Leaves of Diospyros kaki Attenuates D-Galactose-Induced Oxidative Stress and Neuroinflammation-Mediated Brain Aging in Mice.

Authors - Yingjuan Ma
ratWB

Transl Neurosci

Fangchinoline Ameliorates the Expressions of Angiogenic Molecule in Cerebral Ischemia Induced Neuronal Degeneration in Neonatal Rats.

Authors - Han Daicheng
mouseIHC

Sci Rep

Ghrelin protects against contact dermatitis and psoriasiform skin inflammation by antagonizing TNF-α/NF-κB signaling pathways.

Authors - Ruize Qu
mouseWB

Oxid Med Cell Longev

Role of Antioxidants in the Protection from Aging-Related Diseases.

Authors - Daria Maria Monti

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Xie (Verified Customer) (10-06-2021)

Works nice for WB assay.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: RAW 264.7
iNOS Antibody Western Blot validation (1:500 dilution) in RAW 264.7 (Cat no:18985-1-AP)
FH

Prasanth (Verified Customer) (08-19-2019)

This is one of the cleanest and clarity blot I ever got for iNOS. Amazing clarity and no non specific bands. I recommend this for iNOS detection by western blot.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Mouse Fibroblast Cells
FH

Elena (Verified Customer) (08-14-2019)

We tried this antibody with 2 cell lines and it worked just fine. We will use this in the future for other cell lines as well. The antibody produced strong bands at the suggested concentration and we diluted it further.

  • Applications: Western Blot,