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  • KD/KO Validated

iNOS Polyclonal antibody

iNOS Polyclonal Antibody for WB, IF, FC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (3)





Cat no : 18985-1-AP


HEP NOS, Hepatocyte NOS, Inducible NO synthase, Inducible NOS, INOS, NOS, NOS type II, NOS2, NOS2A

Tested Applications

Positive WB detected inmouse liver tissue, rat liver tissue
Positive IF detected inHepG2 cells, HeLa cells
Positive FC detected inHepG2 cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:4000
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18985-1-AP targets iNOS in WB, IP, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, chicken, pig, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name nitric oxide synthase 2, inducible
Calculated Molecular Weight 131 kDa
Observed Molecular Weight 110-130 kDa, 65-70 kDa
GenBank Accession NumberNM_000625
Gene Symbol NOS2
Gene ID (NCBI) 4843
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NOS2, also named as iNOS and NOS2A, produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. NO is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission, antimicrobial and antitumoral activities. NOS2 is a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. iNOS has a very short half-life due to rapid degradation by calpain. iNOS monomer is a direct substrate of calpain I and can be cleaved by calpain I at the canonical CaM-binding site(503-532aa) of iNOS, and then a ~70 kDa band can be detected by western (PMID:11786228). This antibody is specific to NOS2.


Product Specific Protocols
WB protocol for iNOS antibody 18985-1-APDownload protocol
IF protocol for iNOS antibody 18985-1-APDownload protocol
FC protocol for iNOS antibody 18985-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Yasuyo (Verified Customer) (03-01-2022)

This is one of the best IHC signaling I ever got for iNOS. Amazing image. I recommend this for iNOS detection by IHC.

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1:250
  • Cell Tissue Type: skin
iNOS Antibody Immunohistochemistry validation (1:250 dilution) in skin (Cat no:18985-1-AP)

Xie (Verified Customer) (10-06-2021)

Works nice for WB assay.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: RAW 264.7
iNOS Antibody Western Blot validation (1:500 dilution) in RAW 264.7 (Cat no:18985-1-AP)

Prasanth (Verified Customer) (08-19-2019)

This is one of the cleanest and clarity blot I ever got for iNOS. Amazing clarity and no non specific bands. I recommend this for iNOS detection by western blot.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Mouse Fibroblast Cells

Elena (Verified Customer) (08-14-2019)

We tried this antibody with 2 cell lines and it worked just fine. We will use this in the future for other cell lines as well. The antibody produced strong bands at the suggested concentration and we diluted it further.

  • Applications: Western Blot,