iNOS Polyclonal antibody

• 

  WB analysis using 18985-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18985-1-AP (iNOS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 18985-1-AP

  WB result of iNOS antibody (18985-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-iNOS transfected HeLa cells.

• 

  WB analysis of HeLa using 18985-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18985-1-AP (iNOS antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 18985-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using iNOS antibody (18985-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 18985-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 18985-1-AP (iNOS antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HepG2 using 18985-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human iNOS (18985-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• Featured Product
• KD/KO Validated

iNOS Polyclonal Antibody for WB, IF/ICC, FC (Intra), ELISA

Cat No. 18985-1-AP

Publications(812)

Reviews (5)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IF/ICC, FC (Intra), IP, ELISA

Synonyms

NOS2, EC:1.14.13.39, Hepatocyte NOS, HEP-NOS, Inducible NO synthase

---

Tested Applications

• Positive WB detected in: mouse liver tissue, rat liver tissue
• Positive IF/ICC detected in: HepG2 cells, HeLa cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 613 publications below
• IF: See 241 publications below
• IP: See 1 publications below
• ELISA: See 2 publications below
• FC: See 2 publications below

Product Information

18985-1-AP targets iNOS in WB, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, chicken, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: nitric oxide synthase 2, inducible
• Calculated Molecular Weight: 131 kDa
• Observed Molecular Weight: 110-130 kDa, 65-70 kDa
• GenBank Accession Number: NM_000625
• Gene Symbol: iNOS
• Gene ID (NCBI): 4843
• RRID: AB_2782960
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P35228
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NOS2, also named as iNOS and NOS2A, produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. NO is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission, antimicrobial and antitumoral activities. NOS2 is a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. iNOS has a very short half-life due to rapid degradation by calpain. iNOS monomer is a direct substrate of calpain I and can be cleaved by calpain I at the canonical CaM-binding site(503-532aa) of iNOS, and then a ~70 kDa band can be detected by western (PMID:11786228). This antibody is specific to NOS2.

Publications

Species	Application	Title
mouse	WB	Adv Mater; Biomimetic Immunosuppressive Exosomes that Inhibit Cytokine Storms Contribute to the Alleviation of Sepsis.; Authors - Yinan Li
mouse,rat	IF	Bioact Mater; Reprogramming macrophages via immune cell mobilized hydrogel microspheres for osteoarthritis treatments; Authors - Pengcheng Xiao
mouse	WB	Adv Sci (Weinh); 3D Printing of a Vascularized Mini-Liver Based on the Size-Dependent Functional Enhancements of Cell Spheroids for Rescue of Liver Failure; Authors - Jiabin Zhang
human	WB	J Extracell Vesicles; Extracellular vesicle-mediated delivery of circDYM alleviates CUS-induced depressive-like behaviours.; Authors - Xiaoyu Yu
human,mouse	WB	J Exp Clin Cancer Res; Chemotherapy-elicited extracellular vesicle CXCL1 from dying cells promotes triple-negative breast cancer metastasis by activating TAM/PD-L1 signaling; Authors - Shengqi Wang
mouse	WB	Carbohydr Polym; Carboxymethyl chitosan and sodium alginate oxide pH-sensitive dual-release hydrogel for diabetes wound healing: The combination of astilbin liposomes and diclofenac sodium; Authors - Xiaoyu Wu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Marion (Verified Customer) (11-24-2024)

DAPI (blue) + iNOS (red) We expected iNOS staining on microglia cells. However, only nuclei of CA1 neurons were stained here. After discussion with the support, we came to the joint conclusion that the antibody must surely cross-link with the nNOS protein. We tested dilutions of 1/100, 1/200, 1/400, 1/600, 1/800 and 1/1000.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1/200
• Cell Tissue Type: Mouse Brain

iNOS Antibody Immunofluorescence validation (1/200 dilution) in Mouse Brain (Cat no:18985-1-AP)

FH

Yasuyo (Verified Customer) (03-01-2022)

This is one of the best IHC signaling I ever got for iNOS. Amazing image. I recommend this for iNOS detection by IHC.

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:250
• Cell Tissue Type: skin

iNOS Antibody Immunohistochemistry validation (1:250 dilution) in skin (Cat no:18985-1-AP)

FH

Xie (Verified Customer) (10-06-2021)

Works nice for WB assay.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: RAW 264.7

iNOS Antibody Western Blot validation (1:500 dilution) in RAW 264.7 (Cat no:18985-1-AP)

FH

Prasanth (Verified Customer) (08-19-2019)

This is one of the cleanest and clarity blot I ever got for iNOS. Amazing clarity and no non specific bands. I recommend this for iNOS detection by western blot.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse Fibroblast Cells

FH

Elena (Verified Customer) (08-14-2019)

We tried this antibody with 2 cell lines and it worked just fine. We will use this in the future for other cell lines as well. The antibody produced strong bands at the suggested concentration and we diluted it further.

• Applications: Western Blot,