CoraLite® Plus 647-conjugated iNOS Polyclonal antibody

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• CoraLite® Plus 647-conjugated iNOS Polyclonal antibody (CL647-18985)

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• KD/KO Validated

iNOS Polyclonal Antibody for IF/ICC, FC (Intra)

Cat No. CL647-18985 Copied!

Publications (11)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF/ICC, FC (Intra)

Synonyms

NOS2, EC:1.14.13.39, Hepatocyte NOS, HEP-NOS, Inducible NO synthase

• 

  IF Staining of HepG2 using CL647-18985

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CoraLite® Plus 647 iNOS antibody (CL647-18985) at dilution of 1:100.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of HepG2 using CL647-18985

  1X10^6 HepG2 cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human iNOS (CL647-18985) (red), or 0.2 ug isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-18985 targets iNOS in IHC, IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: nitric oxide synthase 2, inducible
• Calculated Molecular Weight: 131 kDa
• Observed Molecular Weight: 110-130 kDa, 65-70 kDa
• GenBank Accession Number: NM_000625
• Gene Symbol: iNOS
• Gene ID (NCBI): 4843
• RRID: AB_2934933
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Excitation Laser: Red Laser (633 nm)
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P35228
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

NOS2, also named as iNOS and NOS2A, produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. NO is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission, antimicrobial and antitumoral activities. NOS2 is a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. iNOS has a very short half-life due to rapid degradation by calpain. iNOS monomer is a direct substrate of calpain I and can be cleaved by calpain I at the canonical CaM-binding site(503-532aa) of iNOS, and then a ~70kD band can be detected by western (PMID:11786228). This antibody is specific to NOS2.

Publications

What published studies show

The iNOS (NOS2) polyclonal antibody (CL647-18985) has 11 citations. These appear in journals including Advanced Science, Biomarker Research, Cell Reports Medicine, Molecular Therapy Oncology, Respiratory Research, International Immunopharmacology, Genes and Immunity, and Biomaterials. Research fields span spinal cord injury regeneration, acute lung injury, myocardial repair, tumor immunotherapy, CAR-T cell therapy, macrophage polarization, rheumatoid arthritis, gastric cancer, and bone regeneration.

Automatically generated from the 11 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
rat	IHC	Biomark Res; Integrating single-cell RNA sequencing and spatial multi-omics reveals the molecular signature of regeneration after spinal cord injury.; Authors - Haoru Dong
		Adv Sci (Weinh); A Pathology-Instructed Theranostic Platform with Mechanoadaptive and ROS-Powered Nanobreathing Functions for Precision Myocardial Repair.; Authors - Zheng Luo
	IF	Adv Sci (Weinh); Natural Enzyme-Loaded Polymeric Stealth Coating-Armed Engineered Probiotics by Disrupting Tumor Lactate Homeostasis to Synergistic Metabolism-Immuno-Enzyme Dynamic Therapy; Authors - Liuzhou Mao
mouse	IF	Adv Sci (Weinh); ROS-Driven Nanoventilator for MRSA-Induced Acute Lung Injury Treatment via In Situ Oxygen Supply, Anti-Inflammation and Immunomodulation; Authors - Zheng Luo
human		Cell Rep Med; Identification and characterization of bone marrow plasma cells producing IFN-γ-neutralizing autoantibodies in adult-onset immunodeficiency.; Authors - Han Wang
human	IF	Mol Ther Oncol; Intrinsic immunosuppressive features of monocytes suppress CAR-T19 through IL-1 pathway modulation in mantle cell lymphoma; Authors - Kun Yun