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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated iNOS Polyclonal antibody

iNOS Polyclonal Antibody for FC (Intra), IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-18985

Synonyms

HEP NOS, Hepatocyte NOS, Inducible NO synthase, Inducible NOS, INOS, NOS, NOS type II, NOS2, NOS2A

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated iNOS Polyclonal anti (CL488-18985)

    IF Staining of HeLa using CL488-18985

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 iNOS antibody (CL488-18985) at dilution of 1:100.Blue (DAPI).

  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite® Plus 488-conjugated iNOS Polyclonal anti (CL488-18985)

    FC experiment of HepG2 using CL488-18985

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human iNOS (CL488-18985) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF detected inHeLa cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-18985 targets iNOS in IF, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name nitric oxide synthase 2, inducible
Calculated Molecular Weight 131 kDa
Observed Molecular Weight 110-130 kDa, 65-70 kDa
GenBank Accession NumberNM_000625
Gene Symbol NOS2
Gene ID (NCBI) 4843
RRIDAB_2919161
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NOS2, also named as iNOS and NOS2A, produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. NO is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission, antimicrobial and antitumoral activities. NOS2 is a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. iNOS has a very short half-life due to rapid degradation by calpain. iNOS monomer is a direct substrate of calpain I and can be cleaved by calpain I at the canonical CaM-binding site(503-532aa) of iNOS, and then a ~70kD band can be detected by western (PMID:11786228). This antibody is specific to NOS2.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 iNOS antibody CL488-18985Download protocol
FC protocol for CL Plus 488 iNOS antibody CL488-18985Download protocol
Standard Protocols
Click here to view our Standard Protocols