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CoraLite® Plus 488-conjugated iNOS Polyclonal antibody
iNOS Polyclonal Antibody for FC (Intra), IF
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF, FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
Cat no : CL488-18985
Synonyms
Validation Data Gallery
Tested Applications
Positive IF detected in | HeLa cells |
Positive FC detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
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Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Product Information
CL488-18985 targets iNOS in IF, FC (Intra) applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Peptide |
Full Name | nitric oxide synthase 2, inducible |
Calculated molecular weight | 131 kDa |
Observed molecular weight | 110-130 kDa, 65-70 kDa |
GenBank accession number | NM_000625 |
Gene symbol | NOS2 |
Gene ID (NCBI) | 4843 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
NOS2, also named as iNOS and NOS2A, produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. NO is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission, antimicrobial and antitumoral activities. NOS2 is a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. iNOS has a very short half-life due to rapid degradation by calpain. iNOS monomer is a direct substrate of calpain I and can be cleaved by calpain I at the canonical CaM-binding site(503-532aa) of iNOS, and then a ~70kD band can be detected by western (PMID:11786228). This antibody is specific to NOS2.
Protocols
Product Specific Protocols | |
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IF protocol for CL Plus 488 iNOS antibody CL488-18985 | Download protocol |
FC protocol for CL Plus 488 iNOS antibody CL488-18985 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |