Tested Applications
| Positive IF/ICC detected in | HeLa cells |
| Positive FC (Intra) detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL594-18985 targets iNOS in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | nitric oxide synthase 2, inducible |
| Calculated Molecular Weight | 131 kDa |
| Observed Molecular Weight | 110-130 kDa, 65-70 kDa |
| GenBank Accession Number | NM_000625 |
| Gene Symbol | iNOS |
| Gene ID (NCBI) | 4843 |
| RRID | AB_2919849 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
| Excitation Laser | Yellow-Green Laser (561 nm) |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P35228 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
NOS2, also named as iNOS and NOS2A, produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. NO is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission, antimicrobial and antitumoral activities. NOS2 is a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. iNOS has a very short half-life due to rapid degradation by calpain. iNOS monomer is a direct substrate of calpain I and can be cleaved by calpain I at the canonical CaM-binding site(503-532aa) of iNOS, and then a ~70kD band can be detected by western (PMID:11786228). This antibody is specific to NOS2.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CL594 iNOS antibody CL594-18985 | Download protocol |
| IF protocol for CL594 iNOS antibody CL594-18985 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
CL594-18985 is a CoraLite® Plus 594-conjugated iNOS (NOS2) polyclonal antibody with 9 citations. These appear in journals including Nature Communications, Biomaterials, Journal of Ethnopharmacology, Biomaterials Advances, International Immunopharmacology, Journal of Biological Chemistry, and Molecular Immunology. Research fields span pulmonary fibrosis, osteoarthritis and cartilage repair, wound healing, acute lung injury, bone regeneration, hepatocellular carcinoma, diabetic wound healing, and cancer immunotherapy.
| Species | Application | Title |
|---|---|---|
Nat Commun Targeted immunotherapy rescues pulmonary fibrosis by reducing activated fibroblasts and regulating alveolar cell profile | ||
Biomaterials Self-supported DNA hydrogel facilitates microenvironment remodeling and cartilage repair to prevent osteoarthritis progression via an ambidextrous strategy. | ||
Biomaterials Magnesium/gallic acid bioMOFs laden carbonized mushroom aerogel effectively heals biofilm-infected skin wounds | ||
J Ethnopharmacol Elsholtzia bodinieri Vaniot ameliorated acute lung injury in mice by regulating pyroptosis, inflammation, oxidative stress and macrophage polarization | ||
Biomater Adv Immunomodulatory and bone regenerative properties of copper/procyanidins-modified titanium surfaces | ||
Int Immunopharmacol miR146a/circKMT2c regulates inflammation in fracture-induced acute lung injury via p65 nuclear translocation |
