NOTCH1 Polyclonal antibody, PBS Only

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  WB analysis using 20687-1-AP (same clone as 20687-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20687-1-AP (NOTCH1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20687-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 20687-1-AP (same clone as 20687-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20687-1-AP (NOTCH1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20687-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 20687-1-AP (same clone as 20687-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20687-1-AP (NOTCH1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20687-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 20687-1-AP (same clone as 20687-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20687-1-AP (NOTCH1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20687-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 20687-1-AP (same clone as 20687-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20687-1-AP (NOTCH1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20687-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 20687-1-AP (same clone as 20687-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20687-1-AP (NOTCH1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20687-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HEK-293 using 20687-1-AP (same clone as 20687-1-PBS)

  IP result of anti-NOTCH1 (IP:20687-1-AP, 4ug; Detection:20687-1-AP 1:600) with HEK-293 cells lysate 1480 ug. This data was developed using the same antibody clone with 20687-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HepG2 using 20687-1-AP (same clone as 20687-1-PBS)

  IP result of anti-NOTCH1 (IP:20687-1-AP, 4ug; Detection:20687-1-AP 1:500) with HepG2 cells lysate 1360 ug. This data was developed using the same antibody clone with 20687-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 20687-1-AP (same clone as 20687-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20687-1-AP (NOTCH1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20687-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 20687-1-AP (same clone as 20687-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20687-1-AP (NOTCH1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20687-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 20687-1-AP (same clone as 20687-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 20687-1-AP (NOTCH1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20687-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 20687-1-AP (same clone as 20687-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 20687-1-AP (NOTCH1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20687-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 20687-1-AP (same clone as 20687-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 20687-1-AP (NOTCH1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20687-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lymphoma using 20687-1-AP (same clone as 20687-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 20687-1-AP (NOTCH1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20687-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lymphoma using 20687-1-AP (same clone as 20687-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 20687-1-AP (NOTCH1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20687-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 20687-1-AP (same clone as 20687-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NOTCH1 antibody (20687-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 20687-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

NOTCH1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 20687-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

hN1, Notch 1, TAN1, Translocation-associated notch protein TAN-1

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Product Information

20687-1-PBS targets NOTCH1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: Notch homolog 1, translocation-associated (Drosophila)
• Calculated Molecular Weight: 273 kDa
• Observed Molecular Weight: 273-300 kDa, 120 kDa
• GenBank Accession Number: NM_017617
• Gene Symbol: NOTCH1
• Gene ID (NCBI): 4851
• RRID: AB_10700012
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P46531
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

NOTCH1, also named as TAN1, belongs to the NOTCH family. NOTCH1 functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBP-J kappa and activates genes of the enhancer of split locus. NOTCH1 affects the implementation of differentiation, proliferation and apoptotic programs. It may be important for normal lymphocyte function. In altered form, may contribute to transformation or progression in some T-cell neoplasms. NOTCH1 is involved in the maturation of both CD4+ and CD8+ cells in the thymus. May be important for follicular differentiation and possibly cell fate selection within the follicle. During cerebellar development, may function as a receptor for neuronal DNER and may be involved in the differentiation of Bergmann glia. Defects in NOTCH1 are a cause of bicuspid aortic valve (BAV).

Notch is synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase (S1 cleavage) in the trans-golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase (S3 cleavage) to release the intracellular domain (NICD) from the membrane. The antibody is specific to NOTCH1. It can recognize the full length NOTCH1(270 kDa) and cleaved NOTCH1 form (120 kDa).