NPB Polyclonal antibody

NPB Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 25517-1-AP

Synonyms

hL7 Neuropeptide B 29, hL7C, hPPL7, neuropeptide B, NPB, PPL7, PPNPB, Preproprotein L7

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Validation Data Gallery

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  IHC staining of human brain using 25517-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25517-1-AP (NPB Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 25517-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25517-1-AP (NPB Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human colon cancer using 25517-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25517-1-AP (NPB Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 25517-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25517-1-AP (NPB Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive IHC detected in: human brain tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25517-1-AP is NPB Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NPB fusion protein Ag21877
• Full Name: neuropeptide B
• Calculated Molecular Weight: 125 aa, 13 kDa
• Observed Molecular Weight: ~10 kDa
• GenBank Accession Number: BC126128
• Gene Symbol: NPB
• Gene ID (NCBI): 256933
• RRID: AB_2880114
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Neuropeptide B, also named as NPB, PPL7 and PPNBP, can be cleaved into two chains, Neuropeptide B-23 (NPB23, hL7) and Neuropeptide B-29 (NPB29, hL7C). It is involved in the regulation of feeding, neuroendocrine system, memory, learning and in the afferent pain pathway. It is widely expressed in central nervous system.