NPHP3 Polyclonal antibody

NPHP3 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat, canine





Cat no : 22026-1-AP


KIAA2000, MKS7, Nephrocystin 3, NPHP3

Tested Applications

Positive WB detected inmouse testis tissue, HepG2 cells, HEK-293 cells, mouse liver tissue, human testis tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhTERT-RPE1 cells, MDCK cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22026-1-AP targets NPHP3 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat, canine samples.

Tested Reactivity human, mouse, rat, canine
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NPHP3 fusion protein Ag16888
Full Name nephronophthisis 3 (adolescent)
Calculated Molecular Weight 1330 aa, 151 kDa
Observed Molecular Weight 145-151 kDa
GenBank Accession NumberBC068082
Gene Symbol NPHP3
Gene ID (NCBI) 27031
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.


Product Specific Protocols
WB protocol for NPHP3 antibody 22026-1-APDownload protocol
IHC protocol for NPHP3 antibody 22026-1-APDownload protocol
IF protocol for NPHP3 antibody 22026-1-APDownload protocol
IP protocol for NPHP3 antibody 22026-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Nat Methods

Genetically encoded calcium indicator illuminates calcium dynamics in primary cilia.

Authors - Su Steven S

Am J Hum Genet

ARL3 Mutations Cause Joubert Syndrome by Disrupting Ciliary Protein Composition.

Authors - Sumaya Alkanderi

Hum Mol Genet

Translational read-through of the RP2 Arg120stop mutation in patient iPSC-derived retinal pigment epithelial cells.

Authors - Nele Schwarz

Int J Mol Sci

Ciliary Proteins Repurposed by the Synaptic Ribbon: Trafficking Myristoylated Proteins at Rod Photoreceptor Synapses.

Authors - Shweta Suiwal

Mol Biol Cell

Interactions between TULP3 tubby domain and ARL13B amphipathic helix promote lipidated protein transport to cilia

Authors - Vivek Reddy Palicharla

Methods Cell Biol

Single-molecule imaging in the primary cilium

Authors - Lucien E Weiss


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Lelongt (Verified Customer) (12-10-2021)

Immunofluorescence is observed in mice cilia but is not restricted to the inversin compartment. Dilution has to be adjusted for WB since there are few bands.

  • Applications: Western Blot, Immunohistochemistry
  • Primary Antibody Dilution: 1/100 immunofluoresecence mice. 1/1000 WB dog
  • Cell Tissue Type: mouce parrafin sections (immunoflorescence). MDCK cells (Western blot)