NPHP3 Polyclonal antibody

NPHP3 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, Canidae

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

5

Reviews (1)

Cat no : 22026-1-AP

Synonyms

KIAA2000, MKS7, Nephrocystin 3, NPHP3

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Validation Data Gallery

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  WB analysis using 22026-1-AP

  mouse testis lysates were subjected to SDS PAGE followed by western blot with 22026-1-AP (NPHP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 22026-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 22026-1-AP (NPHP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 22026-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 22026-1-AP (NPHP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 22026-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 22026-1-AP (NPHP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 22026-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22026-1-AP (NPHP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse testis using 22026-1-AP

  IP Result of anti-NPHP3 (IP:22026-1-AP, 4ug; Detection:22026-1-AP 1:1000) with mouse testis tissue lysate 4000ug.

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  IHC staining of human heart using 22026-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 22026-1-AP (NPHP3 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 22026-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 22026-1-AP (NPHP3 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of hTERT-RPE1 cells using 22026-1-AP

  IF result (the base and the lower half of cilia stain) of anti-NPHP3 (22026-1-AP, 1:50) with serum-starved hTERT-RPE1 (MeOH fixed) by Dr. Moshe Kim.

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  IF Staining of MDCK using 22026-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed MDCK cells using 22026-1-AP (NPHP3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse testis tissue, HepG2 cells, HEK-293 cells, mouse liver tissue, human testis tissue
• Positive IP detected in: mouse testis tissue
• Positive IHC detected in: human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: hTERT-RPE1 cells, MDCK cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• IF: See 5 publications below

Product Information

The immunogen of 22026-1-AP is NPHP3 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat, Canidae
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NPHP3 fusion protein Ag16888
• Full Name: nephronophthisis 3 (adolescent)
• Calculated molecular weight: 1330 aa, 151 kDa
• Observed molecular weight: 145-151 kDa
• GenBank accession number: BC068082
• Gene symbol: NPHP3
• Gene ID (NCBI): 27031
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	IF	Nat Methods; Genetically encoded calcium indicator illuminates calcium dynamics in primary cilia.; Authors - Su Steven S
human	IF	Am J Hum Genet; ARL3 Mutations Cause Joubert Syndrome by Disrupting Ciliary Protein Composition.; Authors - Sumaya Alkanderi
human	IF	Hum Mol Genet; Translational read-through of the RP2 Arg120stop mutation in patient iPSC-derived retinal pigment epithelial cells.; Authors - Nele Schwarz
mouse	IF	Int J Mol Sci; Ciliary Proteins Repurposed by the Synaptic Ribbon: Trafficking Myristoylated Proteins at Rod Photoreceptor Synapses.; Authors - Shweta Suiwal
mouse	IF	Mol Biol Cell; Interactions between TULP3 tubby domain and ARL13B amphipathic helix promote lipidated protein transport to cilia; Authors - Vivek Reddy Palicharla

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Lelongt (Verified Customer) (12-10-2021)

Immunofluorescence is observed in mice cilia but is not restricted to the inversin compartment. Dilution has to be adjusted for WB since there are few bands.

• Applications: Western Blot, Immunohistochemistry
• Primary Antibody Dilution: 1/100 immunofluoresecence mice. 1/1000 WB dog
• Cell Tissue Type: mouce parrafin sections (immunoflorescence). MDCK cells (Western blot)