NPHP3 Polyclonal antibody
NPHP3 Polyclonal Antibody for IF, IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
human, mouse, rat, Canidae
WB, IP, IHC, IF, ELISA
Cat no : 22026-1-AP
Validation Data Gallery
|Positive WB detected in||mouse testis tissue, HepG2 cells, HEK-293 cells, mouse liver tissue, human testis tissue|
|Positive IP detected in||mouse testis tissue|
|Positive IHC detected in||human heart tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||hTERT-RPE1 cells, MDCK cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
The immunogen of 22026-1-AP is NPHP3 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat, Canidae|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||NPHP3 fusion protein Ag16888|
|Full Name||nephronophthisis 3 (adolescent)|
|Calculated molecular weight||1330 aa, 151 kDa|
|Observed molecular weight||145-151 kDa|
|GenBank accession number||BC068082|
|Gene ID (NCBI)||27031|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
|Product Specific Protocols|
|WB protocol for NPHP3 antibody 22026-1-AP||Download protocol|
|IHC protocol for NPHP3 antibody 22026-1-AP||Download protocol|
|IF protocol for NPHP3 antibody 22026-1-AP||Download protocol|
|IP protocol for NPHP3 antibody 22026-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Genetically encoded calcium indicator illuminates calcium dynamics in primary cilia.
Am J Hum Genet
ARL3 Mutations Cause Joubert Syndrome by Disrupting Ciliary Protein Composition.
Hum Mol Genet
Translational read-through of the RP2 Arg120stop mutation in patient iPSC-derived retinal pigment epithelial cells.
Int J Mol Sci
Ciliary Proteins Repurposed by the Synaptic Ribbon: Trafficking Myristoylated Proteins at Rod Photoreceptor Synapses.
Mol Biol Cell
Interactions between TULP3 tubby domain and ARL13B amphipathic helix promote lipidated protein transport to cilia
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Lelongt (Verified Customer) (12-10-2021)
Immunofluorescence is observed in mice cilia but is not restricted to the inversin compartment. Dilution has to be adjusted for WB since there are few bands.