NPHP3 Polyclonal antibody
NPHP3 Polyclonal Antibody for IF, IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat, Canidae
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 22026-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse testis tissue, HepG2 cells, HEK-293 cells, mouse liver tissue, human testis tissue |
Positive IP detected in | mouse testis tissue |
Positive IHC detected in | human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | hTERT-RPE1 cells, MDCK cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF) | IF : 1:20-1:200 |
Sample-dependent, check data in validation data gallery |
Published Applications
IF | See 5 publications below |
Product Information
The immunogen of 22026-1-AP is NPHP3 Fusion Protein expressed in E. coli.
Tested Reactivity | human, mouse, rat, Canidae |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | NPHP3 fusion protein Ag16888 |
Full Name | nephronophthisis 3 (adolescent) |
Calculated molecular weight | 1330 aa, 151 kDa |
Observed molecular weight | 145-151 kDa |
GenBank accession number | BC068082 |
Gene symbol | NPHP3 |
Gene ID (NCBI) | 27031 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
Product Specific Protocols | |
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WB protocol for NPHP3 antibody 22026-1-AP | Download protocol |
IHC protocol for NPHP3 antibody 22026-1-AP | Download protocol |
IF protocol for NPHP3 antibody 22026-1-AP | Download protocol |
IP protocol for NPHP3 antibody 22026-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nat Methods Genetically encoded calcium indicator illuminates calcium dynamics in primary cilia. | ||
Am J Hum Genet ARL3 Mutations Cause Joubert Syndrome by Disrupting Ciliary Protein Composition. | ||
Hum Mol Genet Translational read-through of the RP2 Arg120stop mutation in patient iPSC-derived retinal pigment epithelial cells. | ||
Int J Mol Sci Ciliary Proteins Repurposed by the Synaptic Ribbon: Trafficking Myristoylated Proteins at Rod Photoreceptor Synapses. | ||
Mol Biol Cell Interactions between TULP3 tubby domain and ARL13B amphipathic helix promote lipidated protein transport to cilia |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Lelongt (Verified Customer) (12-10-2021) | Immunofluorescence is observed in mice cilia but is not restricted to the inversin compartment. Dilution has to be adjusted for WB since there are few bands.
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