NPRL3 Polyclonal antibody

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  WB analysis using 26446-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26446-1-AP (C16orf35 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human cervical cancer using 26446-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 26446-1-AP (NPRL3 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

NPRL3 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 26446-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

C16orf35, 14 gene protein, -14 gene protein, Alpha-globin regulatory element-containing gene protein, CGTHBA

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Tested Applications

• Positive WB detected in: K-562 cells, LNCaP cells, THP-1 cells
• Positive IHC detected in: human cervical cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

26446-1-AP targets NPRL3 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag24045

  Product name: Recombinant human C16orf35 protein

  Source: e coli.-derived, PET30a

  Tag: 6*His

  Domain: 1-390 aa of BC004185

  Sequence: MADGNEGPQSPFHHILPKCKLARDLKEAYDSLCTSGVVRLHINSWLEVSFCLPHKIHYAASSLIPPEAIERSLKAIRPYHALLLLSDEKSLLGELPIDCSPALVRVIKTTSAVKNLQQLAQDADLALLQVFQLAAHLVYWGKAIIIYPLCENNVYMLSPNASVCLYSPLAEQFSHQFPSHDLPSVLAKFSLPVSLSEFRNPLAPAVQETQLIQMVVWMLQRRLLIQLHTYVCLMASPSEEEPRPREDDVPFTARVGGRSLSTPNALSFGSPTSSDDMTLTSPSMDNSSAELLPSGDSPLNQRMTENLLASLSEHERAAILSVPAAQNPEDLRMFARLLHYFRGRHHLEEIMYNENTRRSQLLMLFDKFRSVLVVTTHEDPVIAVFQALLP

• Full Name: chromosome 16 open reading frame 35
• Calculated Molecular Weight: 64 kDa
• Observed Molecular Weight: 63 kDa
• GenBank Accession Number: BC004185
• Gene Symbol: C16orf35
• Gene ID (NCBI): 8131
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q12980
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NPRL3 (Nitrogen Permease Regulator Like 3) is a highly conserved tumor suppressor protein. As a core component of the GATOR1 complex, it serves as a key node in the amino acid sensing pathway and negatively regulates the mTORC1 signaling pathway, thereby controlling cell growth, proliferation, and autophagy. Loss of its function leads to hyperactivation of mTORC1 signaling. In neuroscience, loss-of-function mutations in NPRL3 are an important genetic cause of familial focal epilepsy, such as focal epilepsy with variable foci. In oncology, it is frequently inactivated through epigenetic silencing in various cancers, making it a significant tumor suppressor and a potential biomarker.