NRCAM Polyclonal antibody

NRCAM Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 21608-1-AP

Synonyms

hBravo, KIAA0343, neuronal cell adhesion molecule, Neuronal surface protein Bravo, Ng CAM related, Nr CAM, NRCAM



Tested Applications

Positive WB detected inmouse brain tissue, human brain tissue, HEK-293 cells, rat brain tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 21608-1-AP is NRCAM Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NRCAM fusion protein Ag16237
Full Name neuronal cell adhesion molecule
Calculated molecular weight 1304 aa, 144 kDa
Observed molecular weight 130-136 kDa
GenBank accession numberBC098401
Gene symbol NRCAM
Gene ID (NCBI) 4897
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for NRCAM antibody 21608-1-APDownload protocol
IHC protocol for NRCAM antibody 21608-1-APDownload protocol
IF protocol for NRCAM antibody 21608-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Mol Cell Neurosci

Matrix metalloprotease-mediated cleavage of neural glial-related cell adhesion molecules activates quiescent olfactory stem cells via EGFR.

Authors - Zhen-Huang Chen
humanWB

Mol Cell Proteomics

Application of mass spectrometry profiling to establish brusatol as an inhibitor of global protein synthesis.

Authors - Steffan Vartanian
humanIF

Stem Cell Reports

CD73 Regulates Stemness and Epithelial-Mesenchymal Transition in Ovarian Cancer-Initiating Cells.

Authors - Michela Lupia