NRCAM Polyclonal antibody

NRCAM Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 21608-1-AP

Synonyms

hBravo, KIAA0343, neuronal cell adhesion molecule, Neuronal surface protein Bravo, Ng CAM related, Nr CAM, NRCAM

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Validation Data Gallery

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  WB analysis using 21608-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21608-1-AP (NRCAM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 21608-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 21608-1-AP (NRCAM antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 21608-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 21608-1-AP (NRCAM antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HEK-293 using 21608-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using 21608-1-AP (NRCAM antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse brain tissue, human brain tissue, HEK-293 cells, rat brain tissue
• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

The immunogen of 21608-1-AP is NRCAM Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NRCAM fusion protein Ag16237
• Full Name: neuronal cell adhesion molecule
• Calculated molecular weight: 1304 aa, 144 kDa
• Observed molecular weight: 130-136 kDa
• GenBank accession number: BC098401
• Gene symbol: NRCAM
• Gene ID (NCBI): 4897
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Publications

Species	Application	Title
mouse	IHC	Mol Cell Neurosci; Matrix metalloprotease-mediated cleavage of neural glial-related cell adhesion molecules activates quiescent olfactory stem cells via EGFR.; Authors - Zhen-Huang Chen
human	WB	Mol Cell Proteomics; Application of mass spectrometry profiling to establish brusatol as an inhibitor of global protein synthesis.; Authors - Steffan Vartanian
human	IF	Stem Cell Reports; CD73 Regulates Stemness and Epithelial-Mesenchymal Transition in Ovarian Cancer-Initiating Cells.; Authors - Michela Lupia