Name: NRCAM Rabbit Polyclonal antibody
Brand: Proteintech

Tested Applications

Applications:

WB, IHC, IF, ELISA
Application not tested? Please see our Guarantee

NRCAM Antibody WB human brain tissue 21608-1-AP

human brain tissue were subjected to SDS PAGE followed by western blot with 21608-1-AP(NRCAM antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

NRCAM Antibody IHC human brain tissue 21608-1-AP

Immunohistochemical of paraffin-embedded human brain using 21608-1-AP(NRCAM antibody) at dilution of 1:50 (under 10x lens)

Immunohistochemical of paraffin-embedded human brain using 21608-1-AP(NRCAM antibody) at dilution of 1:50 (under 40x lens)

NRCAM Antibody IF HEK-293 cells 21608-1-AP

Immunofluorescent analysis of ( -20°C Ethanol ) fixed HEK-293 cells using 21608-1-AP(NRCAM antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:

human, mouse, rat

Positive WB detected in:

human brain tissue, HEK-293 cells, mouse brain tissue

Positive IHC detected in:

human brain tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Positive IF detected in:

HEK-293 cells

Recommended dilution:

WB : 1:500-1:1000

IHC : 1:20-1:200

IF : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:

Rabbit

Purification method:

Antigen affinity purification

Isotype:

IgG

Storage:

PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20^oC. Aliquoting is unnecessary for -20^oC storage.

Immunogen Information

Immunogen:

NRCAM fusion protein Ag16237

Full name:

neuronal cell adhesion molecule

Calculated molecular weight:

1304aa,144 kDa

Observed molecular weight:

130-136 kDa

GenBank accession number:

BC098401

Gene ID (NCBI):

4897

Gene symbol

NRCAM

Synonyms

hBravo, KIAA0343, neuronal cell adhesion molecule, Neuronal surface protein Bravo, Ng CAM related, Nr CAM, NRCAM

Showing 1- [2] of [2] Publications

Author:		Steffan Vartanian
Pubmed ID:		26711467
Journal:		Mol Cell Proteomics
Title:		Application of mass spectrometry profiling to establish brusatol as an inhibitor of global protein synthesis.
Application:		WB

Species:		human

Author:		Michela Lupia
Pubmed ID:		29551673
Journal:		Stem Cell Reports
Title:		CD73 Regulates Stemness and Epithelial-Mesenchymal Transition in Ovarian Cancer-Initiating Cells.
Application:		IF

Species:		human

WB Figures

NRCAM Antibody WB HEK-293 cells 21608-1-AP

HEK-293 cells were subjected to SDS PAGE followed by western blot with 21608-1-AP(NRCAM antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

human brain tissue were subjected to SDS PAGE followed by western blot with 21608-1-AP(NRCAM antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

NRCAM Antibody WB mouse brain tissue 21608-1-AP

mouse brain tissue were subjected to SDS PAGE followed by western blot with 21608-1-AP (NRCAM antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours

IHC Figures

Immunohistochemical of paraffin-embedded human brain using 21608-1-AP(NRCAM antibody) at dilution of 1:50 (under 10x lens)

Immunohistochemical of paraffin-embedded human brain using 21608-1-AP(NRCAM antibody) at dilution of 1:50 (under 40x lens)

IF Figures

Immunofluorescent analysis of ( -20°C Ethanol ) fixed HEK-293 cells using 21608-1-AP(NRCAM antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

NRCAM Antibody 2 Publications

Rabbit Polyclonal| Catalog number: 21608-1-AP

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Tested Applications

Protocols

Product-specific protocols

Standard Protocols

Product Information

Immunogen Information

Showing 1- [2] of [2] Publications

WB Figures

IHC Figures

IF Figures

NRCAM Antibody 2 Publications

Rabbit Polyclonal| Catalog number: 21608-1-AP

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Special offer: Add control/tag antibody for $99

Tested Applications

Protocols

Product-specific protocols

Standard Protocols

Product Information

Immunogen Information