NRCAM Polyclonal antibody

NRCAM Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 21608-1-AP

Synonyms

hBravo, KIAA0343, neuronal cell adhesion molecule, Neuronal surface protein Bravo, Ng CAM related, Nr CAM, NRCAM



Tested Applications

Positive WB detected inmouse brain tissue, human brain tissue, HEK-293 cells, rat brain tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 21608-1-AP is NRCAM Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NRCAM fusion protein Ag16237
Full Name neuronal cell adhesion molecule
Calculated molecular weight 1304 aa, 144 kDa
Observed molecular weight 130-136 kDa
GenBank accession numberBC098401
Gene symbol NRCAM
Gene ID (NCBI) 4897
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for NRCAM antibody 21608-1-APDownload protocol
IHC protocol for NRCAM antibody 21608-1-APDownload protocol
IF protocol for NRCAM antibody 21608-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cell Proteomics

Application of mass spectrometry profiling to establish brusatol as an inhibitor of global protein synthesis.

Authors - Steffan Vartanian
humanIF

Stem Cell Reports

CD73 Regulates Stemness and Epithelial-Mesenchymal Transition in Ovarian Cancer-Initiating Cells.

Authors - Michela Lupia