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NRCAM Antibody 2 Publications

Rabbit Polyclonal| Catalog number: 21608-1-AP

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Con: 20 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, ELISA
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Images See all

NRCAM Antibody WB human brain tissue 21608-1-AP

human brain tissue were subjected to SDS PAGE followed by western blot with 21608-1-AP(NRCAM antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

NRCAM Antibody IHC human brain tissue 21608-1-AP

Immunohistochemical analysis of paraffin-embedded human brain using 21608-1-AP(NRCAM antibody) at dilution of 1:50 (under 10x lens)

NRCAM Antibody IHC human brain tissue 21608-1-AP

Immunohistochemical analysis of paraffin-embedded human brain using 21608-1-AP(NRCAM antibody) at dilution of 1:50 (under 40x lens)

NRCAM Antibody IF HEK-293 cells 21608-1-AP

Immunofluorescent analysis of ( -20°C Ethanol ) fixed HEK-293 cells using 21608-1-AP(NRCAM antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:
human, mouse, rat
Positive WB detected in:
human brain tissue, HEK-293 cells, mouse brain tissue
Positive IHC detected in:
human brain tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HEK-293 cells
Recommended dilution:
WB : 1:500-1:1000
IHC : 1:20-1:200
IF : 1:50-1:500
Sample-dependent, check data in Validation tab

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
NRCAM fusion protein Ag16237
Full name:
neuronal cell adhesion molecule
Calculated molecular weight:
1304aa,144 kDa
Observed molecular weight:
130-136 kDa
GenBank accession number:
BC098401
Gene ID (NCBI):
4897
Gene symbol
NRCAM
Synonyms
hBravo, KIAA0343, neuronal cell adhesion molecule, Neuronal surface protein Bravo, Ng CAM related, Nr CAM, NRCAM

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