NuMA Polyclonal antibody

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• NuMA Polyclonal antibody (16607-1-AP)

NuMA Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 16607-1-AP

Publications(4)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, monkey and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

NUMA1, NMP22, NMP-22, Nuclear matrix protein-22, Nuclear mitotic apparatus protein

• 

  WB analysis using 16607-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16607-1-AP (NuMA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human breast cancer using 16607-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16607-1-AP (NuMA antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human breast cancer using 16607-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16607-1-AP (NuMA antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HCT 116 using 16607-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HCT 116 cells using NuMA antibody (16607-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HeLa cells, K-562 cells, NIH/3T3 cells, PC-12 cells, COS-7 cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HCT 116 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16607-1-AP targets NuMA in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

• Tested Reactivity: human, mouse, rat, monkey
• Cited Reactivity: human, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9815

  Product name: Recombinant human NUMA1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 845-1193 aa of BC013023

  Sequence: VESLESLYFTPIPARSQAPLESSLDSLGDVFLDSGRKTRSARRRTTQIINITMTKKLDVEEPDSANSSFYSTRSAPASQASLRATSSTQSLARLGSPDYGNSALLSLPGYRPTTRSSARRSQAGVSSGAPPGRNSFYMGTCQDEPEQLDDWNRIAELQQRNRVCPPHLKTCYPLESRPSLSLGTITDEEMKTGDPQETLRRASMQPIQIAEGTGITTRQQRKRVSLEPHQGPGTPESKKATSCFPRPMTPRDRHEGRKQSTTEAQKKAAPASTKQADRRQSMAFSILNTPKKLGNSLLRRGASKKALSKASPNTRSGTRRSPRIATTTASAATAAAIGATPRAKGKAKH

• Full Name: nuclear mitotic apparatus protein 1
• Calculated Molecular Weight: 238 kDa
• Observed Molecular Weight: 238 kDa
• GenBank Accession Number: BC013023
• Gene Symbol: NuMA
• Gene ID (NCBI): 4926
• RRID: AB_2154616
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q14980
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NUMA1, also named as Nuclear matrix protein-22, is a 2115 amino acid protein, which forms multiarm oligomers by association of C-terminal tail domains. NUMA1 resides in the nuclear matrix during interphase. NUMA1 is a highly abundant component of the nuclear matrix where it may serve a non-mitotic structural role and occupies the majority if the nuclear volume. NUMA1 is required for maintenance and establishment of the mitotic spindle poles during symmetric cell divisions, functioning as a tether linking bulk microtubules of the spindle to centrosomes.

Publications

What published studies show

Automatically generated from the publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
		DNA Repair (Amst); The nuclear structural protein NuMA is required for efficient DNA replication and supports the association of core replication factors to replication forks.; Authors - Zhen-Guo Wang
pig	IF	J Cell Sci; RNA localization to the mitotic spindle is essential for early development and is regulated by kinesin-1 and dynein; Authors - Carolyn M Remsburg
human	WB	bioRxiv; The nuclear structural protein NuMA is required for efficient DNA replication by ensuring association of core replication factors to replication forks.; Authors - Zhen-Guo Wang ; KO Validated
human	WB	Cell Death Dis; CBX2 phase-separation contributes to homologous recombination repair and drug resistance in ovarian cancer.; Authors - Si Sun

Reviews

5.0 out of 5

1 verified customer reviews

What customers say

Automatically generated from the verified customer reviews below by Able AI.

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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Sara (Verified Customer) (12-10-2025)

Performed well in Western Blot and Immunofluorescence, particularly Proximity Ligation Assays in combination with other primary antibodies.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:100 in PLA, 1:800 in WB