Tested Applications
| Positive WB detected in | HeLa cells, K-562 cells, NIH/3T3 cells, PC-12 cells, COS-7 cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HCT 116 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16607-1-AP targets NuMA in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.
| Tested Reactivity | human, mouse, rat, monkey |
| Cited Reactivity | human, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9815 Product name: Recombinant human NUMA1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 845-1193 aa of BC013023 Sequence: VESLESLYFTPIPARSQAPLESSLDSLGDVFLDSGRKTRSARRRTTQIINITMTKKLDVEEPDSANSSFYSTRSAPASQASLRATSSTQSLARLGSPDYGNSALLSLPGYRPTTRSSARRSQAGVSSGAPPGRNSFYMGTCQDEPEQLDDWNRIAELQQRNRVCPPHLKTCYPLESRPSLSLGTITDEEMKTGDPQETLRRASMQPIQIAEGTGITTRQQRKRVSLEPHQGPGTPESKKATSCFPRPMTPRDRHEGRKQSTTEAQKKAAPASTKQADRRQSMAFSILNTPKKLGNSLLRRGASKKALSKASPNTRSGTRRSPRIATTTASAATAAAIGATPRAKGKAKH Predict reactive species |
| Full Name | nuclear mitotic apparatus protein 1 |
| Calculated Molecular Weight | 238 kDa |
| Observed Molecular Weight | 238 kDa |
| GenBank Accession Number | BC013023 |
| Gene Symbol | NuMA |
| Gene ID (NCBI) | 4926 |
| RRID | AB_2154616 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q14980 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
NUMA1, also named as Nuclear matrix protein-22, is a 2115 amino acid protein, which forms multiarm oligomers by association of C-terminal tail domains. NUMA1 resides in the nuclear matrix during interphase. NUMA1 is a highly abundant component of the nuclear matrix where it may serve a non-mitotic structural role and occupies the majority if the nuclear volume. NUMA1 is required for maintenance and establishment of the mitotic spindle poles during symmetric cell divisions, functioning as a tether linking bulk microtubules of the spindle to centrosomes.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for NuMA antibody 16607-1-AP | Download protocol |
| IHC protocol for NuMA antibody 16607-1-AP | Download protocol |
| WB protocol for NuMA antibody 16607-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-NuMA antibody (catalog number 16607-1-AP) has 4 total citations. These appear in Cell Death & Disease, Journal of Cell Science, DNA Repair, and bioRxiv. The associated research fields span ovarian cancer (phase separation and drug resistance), early embryonic development (RNA localization to the mitotic spindle), and DNA replication (NuMA's role in associating core replication factors to replication forks).
| Species | Application | Title |
|---|---|---|
Cell Death Dis CBX2 phase-separation contributes to homologous recombination repair and drug resistance in ovarian cancer. | ||
J Cell Sci RNA localization to the mitotic spindle is essential for early development and is regulated by kinesin-1 and dynein | ||
DNA Repair (Amst) The nuclear structural protein NuMA is required for efficient DNA replication and supports the association of core replication factors to replication forks. | ||
bioRxiv The nuclear structural protein NuMA is required for efficient DNA replication by ensuring association of core replication factors to replication forks.
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Reviews
What customers say
The single review for this product reports excellent performance across multiple applications. The antibody worked well in Western Blot and Immunofluorescence, and was particularly effective in Proximity Ligation Assays (PLA) when combined with other primary antibodies. The reviewer used a dilution of 1:100 for PLA and 1:800 for WB, and awarded the product a 5-star rating. Overall, the feedback is highly positive, highlighting reliable results in both standard and multiplex applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Sara (Verified Customer) (12-10-2025) | Performed well in Western Blot and Immunofluorescence, particularly Proximity Ligation Assays in combination with other primary antibodies.
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