NUMB Monoclonal antibody

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  WB analysis of A431 using 60137-2-Ig

  A431 cells were subjected to SDS PAGE followed by western blot with 60137-2-Ig (NUMB antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 60137-2-Ig

  A431 cells were subjected to SDS PAGE followed by western blot with 60137-2-Ig (NUMB antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 60137-2-Ig

  A431 cells were subjected to SDS PAGE followed by western blot with 60137-2-Ig (NUMB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

NUMB Monoclonal Antibody for ELISA

Cat No. 60137-2-Ig

Clone No.1A8A1

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

ELISA

Synonyms

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Product Information

60137-2-Ig targets NUMB in ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: peptide
• Full Name: numb homolog (Drosophila)
• Calculated Molecular Weight: 71 kDa
• GenBank Accession Number: BC068476
• Gene Symbol: NUMB
• Gene ID (NCBI): 8650
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P49757
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NUMB, also named as S171, plays a role in the determination of cell fates during development. It is a membrane-bound protein that has been shown to associate with EPS15, LNX1, and NOTCH1. The degradation of NUMB is induced in a proteasome-dependent manner by MDM2. Four transcript variants encoding different isoforms have been found for this gene. The antibody can bind to all these four isoforms P65, P66, P71 and P72, can’t recognize the NUMBL.