NUPL2 Polyclonal antibody, PBS Only

• 

  WB analysis of HEK-293 using 16587-1-AP (same clone as 16587-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16587-1-AP (NUPL2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16587-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human liver using 16587-1-AP (same clone as 16587-1-PBS)

  human liver tissue were subjected to SDS PAGE followed by western blot with 16587-1-AP (NUPL2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16587-1-PBS in a different storage buffer formulation.

• 

  WB analysis of L02 using 16587-1-AP (same clone as 16587-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 16587-1-AP (NUPL2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16587-1-PBS in a different storage buffer formulation.

NUPL2 Polyclonal Antibody for WB, Indirect ELISA

Cat No. 16587-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

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Product Information

16587-1-PBS targets NUPL2 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag8816

  Product name: Recombinant human NUPL2 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-224 aa of BC005327

  Sequence: MAICQFFLQGRCRFGDRCWNEHPGARGAGGGRQQPQQQPSGNNRRGWNTTSQRYSNVIQPSSFSKSTPWGGSRDQEKPYFSSFDSGASTNRKEGFGLSENPFASLSPDEQKDEKKLLEGIVKDMEVWESSGQWMFSVYSPVKKKPNISGFTDISPEELRLEYHNFLTSNNLQSYLNSVQRLINQWRNRVNELKSLNISTKVALKCLWKIYTHLSPKIYGPDCAL

• Full Name: nucleoporin like 2
• Calculated Molecular Weight: 45 kDa
• Observed Molecular Weight: 45 kDa, 33-36 kDa
• GenBank Accession Number: BC005327
• Gene Symbol: NUPL2
• Gene ID (NCBI): 11097
• RRID: AB_2267702
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O15504
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

NUPL2 (also known as NLP-1, or hCG1) is an ubiquitous nuclear protein containing many phenylalanine-glycine (FG) repeats. As the functional orthologue of the budding yeast Nup42/Rip1 nucleoporin, NUPL2 is required for the export of mRNAs containing poly(A) tails from the nucleus into the cytoplasm (PMID: 10610322). In case of infection by HIV-1, it may participate in the docking of viral Vpr at the nuclear envelope (PMID: 16000379). NUPL2 localizes to the nuclear envelope and could also be mobile within the nucleus. It promotes CRM1-dependent nuclear protein export (PMID: 22250199).