NUPL2 Polyclonal antibody

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  WB analysis of HEK-293 using 16587-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16587-1-AP (NUPL2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 16587-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 16587-1-AP (NUPL2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 16587-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 16587-1-AP (NUPL2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

NUPL2 Polyclonal Antibody for WB, ELISA

Cat No. 16587-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

CG1, hCG1, NLP 1, NLP_1, NLP1, Nucleoporin hCG1, nucleoporin like 2, Nucleoporin like protein 2, NUP42 homolog, NUPL2

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Tested Applications

• Positive WB detected in: HEK-293 cells, human liver tissue, L02 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

16587-1-AP targets NUPL2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag8816

  Product name: Recombinant human NUPL2 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-224 aa of BC005327

  Sequence: MAICQFFLQGRCRFGDRCWNEHPGARGAGGGRQQPQQQPSGNNRRGWNTTSQRYSNVIQPSSFSKSTPWGGSRDQEKPYFSSFDSGASTNRKEGFGLSENPFASLSPDEQKDEKKLLEGIVKDMEVWESSGQWMFSVYSPVKKKPNISGFTDISPEELRLEYHNFLTSNNLQSYLNSVQRLINQWRNRVNELKSLNISTKVALKCLWKIYTHLSPKIYGPDCAL

• Full Name: nucleoporin like 2
• Calculated Molecular Weight: 45 kDa
• Observed Molecular Weight: 45 kDa, 33-36 kDa
• GenBank Accession Number: BC005327
• Gene Symbol: NUPL2
• Gene ID (NCBI): 11097
• RRID: AB_2267702
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O15504
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NUPL2 (also known as NLP-1, or hCG1) is an ubiquitous nuclear protein containing many phenylalanine-glycine (FG) repeats. As the functional orthologue of the budding yeast Nup42/Rip1 nucleoporin, NUPL2 is required for the export of mRNAs containing poly(A) tails from the nucleus into the cytoplasm (PMID: 10610322). In case of infection by HIV-1, it may participate in the docking of viral Vpr at the nuclear envelope (PMID: 16000379). NUPL2 localizes to the nuclear envelope and could also be mobile within the nucleus. It promotes CRM1-dependent nuclear protein export (PMID: 22250199).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Front Cell Dev Biol; Loss of FAM111B protease mutated in hereditary fibrosing poikiloderma negatively regulates telomere length; Authors - Maciej Kliszczak