NUSAP1 Polyclonal antibody, PBS Only

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  WB analysis using 12024-1-AP (same clone as 12024-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12024-1-AP (NUSAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12024-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 12024-1-AP (same clone as 12024-1-PBS)

  IP result of anti-NUSAP1 (IP:12024-1-AP, 4ug; Detection:12024-1-AP 1:500) with HeLa cells lysate 2000ug. This data was developed using the same antibody clone with 12024-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 12024-1-AP (same clone as 12024-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12024-1-AP (NUSAP1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12024-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 12024-1-AP (same clone as 12024-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12024-1-AP (NUSAP1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12024-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 12024-1-AP (same clone as 12024-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12024-1-AP (NUSAP1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12024-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 12024-1-AP (same clone as 12024-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12024-1-AP (NUSAP1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12024-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 12024-1-AP (same clone as 12024-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NUSAP1 antibody (12024-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12024-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

NUSAP1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 12024-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

ANKT, BM037, BM-037, LNP, Nucleolar and spindle-associated protein 1

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Product Information

12024-1-PBS targets NUSAP1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2654

  Product name: Recombinant human NUSAP1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-440 aa of BC001308

  Sequence: MIIPSLEELDSLKYSDLQNLAKSLGLRANLRATKLLKALKGYIKHEARKGNENQDESQTSASSCDETEIQISNQEEAERQPLGHVTKTRRRCKTVRVDPDSQQNHSEIKISNPTEFQNHEKQESQDLRATAKVPSPPDEHQEAENAVSSGNRDSKVPSEGKKSLYTDESSKPGKNKRTAITTPNFKKLHEAHFKEMESIDQYIERKKKHFEEHNSMNELKQPINKGGVRTPVPPRGRLSVASTPISQRRSQGRSCGPASQSTLGLKGSLKRSAISAAKTGVRFSAATKDNEHKRSLTKTPARKSAHVTVSGGTPKGEAVLGTHKLKTITGNSAAVITPFKLTTEATQTPVSNKKPVFDLKASLSRPLNYEPHKGKLKPWGQSKENNYLNQHVNRINFYKKTYKQPHLQTKEEQRKKREQERKEKKAKVLGMRRGLILAED

• Full Name: nucleolar and spindle associated protein 1
• Calculated Molecular Weight: 440 aa, 49 kDa
• Observed Molecular Weight: 49-60 kDa
• GenBank Accession Number: BC001308
• Gene Symbol: NUSAP1
• Gene ID (NCBI): 51203
• RRID: AB_2157789
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9BXS6
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

NUSAP1, also named as ANKT, belongs to the NUSAP family. It is microtubule-associated protein with the capacity to bundle and stabilize microtubules. NUSAP1 may associate with chromosomes and promote the organization of mitotic spindle microtubules around them. NUSAP1 plays a role in connecting apoptosis with cell migration and it is a potentially involved in morphogenesis in vertebrates (PMID:21203972). NUSAP1 is an immunogenic antigen in 65% of patients with AML following allogeneic HCT and suggests a tumor antigen role(PMID:20053754 ). NuSAP1 protein level is tightly regulated by the APC/C ubiquitin ligase complex and NuSAP induces mitotic arrest dependent of its microtubule affinity (PMID:17618083). NUSAP1 is overexpressed in pituitary adenomas.